Tested Applications
| Positive WB detected in | HEK-293 cells, HuH-7 cells, HepG2 cells, Jurkat cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15131-1-AP targets ATG4B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7261 Product name: Recombinant human ATG4B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-393 aa of BC000719 Sequence: MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVEQQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL Predict reactive species |
| Full Name | ATG4 autophagy related 4 homolog B (S. cerevisiae) |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC000719 |
| Gene Symbol | ATG4B |
| Gene ID (NCBI) | 23192 |
| RRID | AB_2064405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4P1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATG4B antibody 15131-1-AP | Download protocol |
| IP protocol for ATG4B antibody 15131-1-AP | Download protocol |
| WB protocol for ATG4B antibody 15131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATG4B antibody (Cat# 15131-1-AP) has accumulated 37 citations published in journals including Autophagy, Nature Communications, Neuron, Redox Biology, Cell Death & Disease, Cancer Letters, Pharmacological Research, and Free Radical Biology and Medicine. Associated research fields encompass autophagy mechanisms, multiple cancer types (hepatocellular, colorectal, gastric, breast, glioblastoma, AML), neurodegenerative diseases (ALS, frontotemporal dementia), diabetes, toxicology, viral infections, and inflammatory responses.
| Species | Application | Title |
|---|---|---|
Autophagy Lopinavir enhances anoikis by remodeling autophagy in a circRNA-dependent manner | ||
Autophagy RHOD mediates ATG9A trafficking to promote autophagosome formation during autophagy in cancer | ||
Autophagy RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye. | ||
Autophagy ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1
| ||
Nat Commun Targeting chaperone-mediated autophagy inhibits properties of glioblastoma stem cells and restores anti-tumor immunity. |
