ATG5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATG5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

170

Reviews (1)

Cat no : 10181-2-AP

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

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Validation Data Gallery

• 

  WB analysis using 10181-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10181-2-AP

  WB result of ATG5 antibody (10181-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells.

• 

  WB analysis of SH-SY5Y using 10181-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10181-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10181-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10181-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10181-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10181-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10181-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, HEK-293T cells, mouse kidney tissue, human brain tissue, Raji cells, SH-SY5Y cells, Jurkat cells
• Positive IHC detected in: human colon cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 29 publications below
• WB: See 157 publications below
• IHC: See 14 publications below
• IF: See 13 publications below
• FC: See 2 publications below

Product Information

10181-2-AP targets ATG5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, chicken, grouper, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG5 fusion protein Ag0214
• Full Name: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 32 kDa, 40-45 kDa, 50-55 kDa
• GenBank accession number: BC002699
• Gene symbol: ATG5
• Gene ID (NCBI): 9474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Publications

Species	Application	Title
human	WB	Arch Toxicol; PMID: 30659323; Authors - Qian Zhao
mouse	WB	Biochem Biophys Res Commun; Autophagy exerts pivotal roles in regulatory effects of 1α,25-(OH)2D3 on the osteoclastogenesis.; Authors - Lianmei Ji
human	WB	J Cell Physiol; Sodium fluoride regulates the osteo/odontogenic differentiation of stem cells from apical papilla by modulating autophagy.; Authors - Yin Pan
grouper	WB	Front Immunol; Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus.; Authors - Chen Li ; KD Validated
mouse	IF	Mol Psychiatry; Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.; Authors - Md Golam Sharoar
human	IHC	Mol Cancer; Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.; Authors - Qiang Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Benjamin (Verified Customer) (01-07-2020)

Works well in western blot in 3% milk TBS-T

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK239 Mammalian Cell Lysate

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