Validation Data Gallery
|Positive WB detected in||A549 cells, HEK-293T cells, mouse kidney tissue, human brain tissue, Raji cells, HepG2 cells, HeLa cells, SH-SY5Y cells, Jurkat cells|
|Positive IHC detected in||human colon cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse cortical neurons|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10181-2-AP targets ATG5 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, grouper, human, monkey, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATG5 fusion protein Ag0214|
|Full Name||ATG5 autophagy related 5 homolog (S. cerevisiae)|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32 kDa, 40-45 kDa, 50-55 kDa|
|GenBank accession number||BC002699|
|Gene ID (NCBI)||9474|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Benjamin (Verified Customer) (01-07-2020)
Works well in western blot in 3% milk TBS-T