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ATG5 Polyclonal antibody
ATG5 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10181-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, MDA-MB-231 cells, T-47D cells, Raji cells, HeLa cells, mouse kidney tissue, human brain tissue, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse cortical neurons |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 19 publications below |
| WB | See 84 publications below |
| IHC | See 6 publications below |
| IF | See 6 publications below |
| FC | See 1 publications below |
Product Information
10181-2-AP targets ATG5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | grouper, human, monkey, mouse, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG5 fusion protein Ag0214 |
| Full Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 32 kDa, 40-45 kDa, 50-55 kDa |
| GenBank accession number | BC002699 |
| Gene symbol | ATG5 |
| Gene ID (NCBI) | 9474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG5 antibody 10181-2-AP | Download protocol |
| IHC protocol for ATG5 antibody 10181-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Autophagy exerts pivotal roles in regulatory effects of 1α,25-(OH)2D3 on the osteoclastogenesis. | ||
J Cell Physiol Sodium fluoride regulates the osteo/odontogenic differentiation of stem cells from apical papilla by modulating autophagy. | ||
Front Immunol Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus.
| ||
Mol Psychiatry Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. | ||
Mol Cancer Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Benjamin (Verified Customer) (01-07-2020) | Works well in western blot in 3% milk TBS-T
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