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KD/KO Validated

ATG5 Polyclonal antibody

Name: ATG5 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 10181-2-AP
Rating: 4.00 (1 reviews)

ATG5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

126

Reviews (1)

Cat no : 10181-2-AP

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

Validation Data Gallery

WB analysis using 10181-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10181-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10181-2-AP
WB result of ATG5 antibody (10181-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells.
WB analysis of SH-SY5Y using 10181-2-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IF Staining of mouse cortical neurons using 10181-2-AP
IF result of anti-ATG5 (10181-2-AP) with E15 mouse cortical neurons.
IHC staining of human colon cancer using 10181-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 10181-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 10181-2-AP

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Tested Applications

Positive WB detected in	A549 cells, HEK-293T cells, mouse kidney tissue, human brain tissue, Raji cells, HepG2 cells, HeLa cells, SH-SY5Y cells, Jurkat cells
Positive IHC detected in	human colon cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	mouse cortical neurons

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 23 publications below
WB	See 116 publications below
IHC	See 10 publications below
IF	See 11 publications below
FC	See 1 publications below

Product Information

10181-2-AP targets ATG5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	bovine, grouper, human, monkey, mouse, pig, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATG5 fusion protein Ag0214
Full Name	ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated molecular weight	32 kDa
Observed molecular weight	32 kDa, 40-45 kDa, 50-55 kDa
GenBank accession number	BC002699
Gene symbol	ATG5
Gene ID (NCBI)	9474
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Protocols

Product Specific Protocols
WB protocol for ATG5 antibody 10181-2-AP	Download protocol
IHC protocol for ATG5 antibody 10181-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Arch Toxicol PMID: 30659323 Authors - Qian Zhao View Article
mouse	WB	Biochem Biophys Res Commun Autophagy exerts pivotal roles in regulatory effects of 1α,25-(OH)2D3 on the osteoclastogenesis. Authors - Lianmei Ji View Article
human	WB	J Cell Physiol Sodium fluoride regulates the osteo/odontogenic differentiation of stem cells from apical papilla by modulating autophagy. Authors - Yin Pan View Article
grouper	WB	Front Immunol Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus. Authors - Chen Li KD Validated View Article
mouse	IF	Mol Psychiatry Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. Authors - Md Golam Sharoar View Article
human	IHC	Mol Cancer Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy. Authors - Qiang Cai View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Benjamin (Verified Customer) (01-07-2020)

Works well in western blot in 3% milk TBS-T

Applications: Western Blot,
Primary Antibody Dilution: 1:1000
Cell Tissue Type: HEK239 Mammalian Cell Lysate