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ATG5 Polyclonal antibody

ATG5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10181-2-AP

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

Validation Data Gallery

  • WB analysis using 10181-2-AP

    WB analysis using 10181-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10181-2-AP

    WB analysis using 10181-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 10181-2-AP

    WB analysis of HepG2 using 10181-2-AP

    WB result of ATG5 antibody (10181-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells.

  • WB analysis of SH-SY5Y using 10181-2-AP

    WB analysis of SH-SY5Y using 10181-2-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cortical neurons using 10181-2-AP

    IF Staining of mouse cortical neurons using 10181-2-AP

    IF result of anti-ATG5 (10181-2-AP) with E15 mouse cortical neurons.

  • IHC staining of human colon cancer using 10181-2-AP

    IHC staining of human colon cancer using 10181-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 10181-2-AP

    IHC staining of human colon cancer using 10181-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, MDA-MB-231 cells, T-47D cells, Raji cells, HeLa cells, mouse kidney tissue, human brain tissue, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cortical neurons

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10181-2-AP targets ATG5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity grouper, human, monkey, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG5 fusion protein Ag0214
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa, 40-45 kDa, 50-55 kDa
GenBank accession numberBC002699
Gene symbol ATG5
Gene ID (NCBI) 9474
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Protocols

Product Specific Protocols
WB protocol for ATG5 antibody 10181-2-APDownload protocol
IHC protocol for ATG5 antibody 10181-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arch Toxicol

PMID: 30659323

Authors - Qian Zhao
View Article
mouseWB

Biochem Biophys Res Commun

Autophagy exerts pivotal roles in regulatory effects of 1α,25-(OH)2D3 on the osteoclastogenesis.

Authors - Lianmei Ji
View Article
humanWB

J Cell Physiol

Sodium fluoride regulates the osteo/odontogenic differentiation of stem cells from apical papilla by modulating autophagy.

Authors - Yin Pan
View Article
grouperWB

Front Immunol

Fish Autophagy Protein 5 Exerts Negative Regulation on Antiviral Immune Response Against Iridovirus and Nodavirus.

Authors - Chen Li
  • KD Validated
View Article
mouseIF

Mol Psychiatry

Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.

Authors - Md Golam Sharoar
View Article
humanIHC

Mol Cancer

Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.

Authors - Qiang Cai
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Benjamin (Verified Customer) (01-07-2020)

Works well in western blot in 3% milk TBS-T

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK239 Mammalian Cell Lysate