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ATG5 Monoclonal antibody
ATG5 Monoclonal Antibody for FC, IHC, WB, ELISA
Cat no : 66744-1-Ig
Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, HeLa cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells
|Positive IHC detected in
|human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:150-1:600
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66744-1-Ig targets ATG5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, pig
|Host / Isotype
|Mouse / IgG2a
|ATG5 fusion protein Ag16685
|ATG5 autophagy related 5 homolog (S. cerevisiae)
|Calculated molecular weight
|Observed molecular weight
|50-55 and 40-45 kDa
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)
Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells
Cold exposure protects against medial arterial calcification development via autophagy
Reciprocal regulation of NRF2 by autophagy and ubiquitin-proteasome modulates vascular endothelial injury induced by copper oxide nanoparticles.
Cancer Cell Int
LncRNA LZTS1-AS1 induces proliferation, metastasis and inhibits autophagy of pancreatic cancer cells through the miR-532 /TWIST1 signaling pathway
Tetrachlorobenzoquinone exposure triggers ferroptosis contributing to its neurotoxicity.
Stem Cell Res Ther
Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marina (Verified Customer) (11-22-2021)
ATG 5 and the complex ATG5-ATG12 are visible, almost no background.