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  • KD/KO Validated

ATG5 Monoclonal antibody

ATG5 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3B8

Cat no : 66744-1-Ig

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66744-1-Ig targets ATG5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATG5 fusion protein Ag16685
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated molecular weight 32 kDa
Observed molecular weight 50-55 and 40-45 kDa
GenBank accession numberBC002699
Gene symbol ATG5
Gene ID (NCBI) 9474
RRIDAB_2882092
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)

Protocols

Product Specific Protocols
WB protocol for ATG5 antibody 66744-1-IgDownload protocol
IHC protocol for ATG5 antibody 66744-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Gastroenterology

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Authors - Decai Wang
humanWB

J Nanobiotechnology

Cold exposure protects against medial arterial calcification development via autophagy

Authors - Fu-Xing-Zi Li
humanWB

J Nanobiotechnology

Reciprocal regulation of NRF2 by autophagy and ubiquitin-proteasome modulates vascular endothelial injury induced by copper oxide nanoparticles.

Authors - Na Li
  • KO Validated
humanWB

Cancer Cell Int

LncRNA LZTS1-AS1 induces proliferation, metastasis and inhibits autophagy of pancreatic cancer cells through the miR-532 /TWIST1 signaling pathway

Authors - Hui Wu
ratWB

Chemosphere

Tetrachlorobenzoquinone exposure triggers ferroptosis contributing to its neurotoxicity.

Authors - Zixuan Liu
mouseWB

Stem Cell Res Ther

Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.

Authors - Jie Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marina (Verified Customer) (11-22-2021)

ATG 5 and the complex ATG5-ATG12 are visible, almost no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
  • Cell Tissue Type: primary mouse microglia