ATG5 Monoclonal antibody

• Featured Product
• KD/KO Validated

ATG5 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

20

CloneNo.

2D3B8

Reviews (1)

Cat no : 66744-1-Ig

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

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Validation Data Gallery

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  WB analysis of HeLa using 66744-1-Ig

  WB result of ATG5 antibody (66744-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HeLa cells.

• 

  WB analysis using 66744-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66744-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66744-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66744-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66744-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66744-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below

Product Information

66744-1-Ig targets ATG5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG5 fusion protein Ag16685
• Full Name: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 50-55 and 40-45 kDa
• GenBank accession number: BC002699
• Gene symbol: ATG5
• Gene ID (NCBI): 9474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)

Publications

Species	Application	Title
human	WB	J Nanobiotechnology; Reciprocal regulation of NRF2 by autophagy and ubiquitin-proteasome modulates vascular endothelial injury induced by copper oxide nanoparticles.; Authors - Na Li ; KO Validated
rat	WB	Chemosphere; Tetrachlorobenzoquinone exposure triggers ferroptosis contributing to its neurotoxicity.; Authors - Zixuan Liu
mouse	WB	Stem Cell Res Ther; Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.; Authors - Jie Zhou
human,mouse	WB	iScience; A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.; Authors - Sheng Li ; KD Validated
mouse	WB	Eur J Immunol; ATG16L2 inhibits NLRP3 inflammasome activation through promoting ATG5-12-16L1 complex assembly and autophagy.; Authors - Dongyang Wang
mouse	WB	Front Pharmacol; Selective STAT3 Inhibitor Alantolactone Ameliorates Osteoarthritis via Regulating Chondrocyte Autophagy and Cartilage Homeostasis; Authors - Wenbin Pei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (11-22-2021)

ATG 5 and the complex ATG5-ATG12 are visible, almost no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
• Cell Tissue Type: primary mouse microglia