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ATP1A3 (C-terminal) Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 10868-1-AP

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

ATP1A3 (C-terminal) Antibody WB C2C12 cell 10868-1-AP

C2C12 cell were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

ATP1A3 (C-terminal) Antibody IHC human cerebellum tissue 10868-1-AP

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
C2C12 cell
Positive IHC detected in:
human cerebellum tissue, mouse brain tissue, mouse skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:3000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATP1A3 (C-terminal) fusion protein Ag1313
Full name:
ATPase, Na+/K+ transporting, alpha 3 polypeptide
Calculated molecular weight:
113 kDa
Observed molecular weight:
100-113 kDa
GenBank accession number:
BC015566
Gene ID (NCBI):
478
Gene symbol
ATP1A3
Synonyms
ATP1A3, DYT12, RDP, Sodium pump subunit alpha 3

Background

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na+/K+ homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)


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