|Positive WB detected in||HeLa cells, A431 cells, human brain tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13310-1-AP targets ATP2C1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP2C1 fusion protein Ag4143|
|Full Name||ATPase, Ca++ transporting, type 2C, member 1|
|Calculated molecular weight||919 aa, 101 kDa|
|Observed molecular weight||95-103 kDa|
|GenBank accession number||BC028139|
|Gene ID (NCBI)||27032|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (09-12-2019)
This antibody could detect endogenous ATP2C1 both for WB and IF. For WB, besides the band at the expected molecular weight, it also gives a non specific band at ~ 150 kd.