ATP2C1 Polyclonal antibody

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  WB analysis of HeLa using 13310-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 13310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 13310-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13310-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 13310-1-AP

  IP result of anti-ATP2C1 (IP:13310-1-AP, 4ug; Detection:13310-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse brain using 13310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13310-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP2C1 antibody (13310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ATP2C1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13310-1-AP

Publications(6)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PMR1, HUSSY-28, hSPCA1, EC:7.2.2.10, Calcium-transporting ATPase type 2C member 1

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, human brain tissue, rat brain tissue, mouse brain tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below

Product Information

13310-1-AP targets ATP2C1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP2C1 fusion protein Ag4143
• Full Name: ATPase, Ca++ transporting, type 2C, member 1
• Calculated Molecular Weight: 919 aa, 101 kDa
• Observed Molecular Weight: 95-103 kDa
• GenBank Accession Number: BC028139
• Gene Symbol: ATP2C1
• Gene ID (NCBI): 27032
• RRID: AB_10640808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P98194
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2C1, also known as hSPCA1, belongs to the family of P-type cation transport ATPases. ATP2C1 is a Golgi-localized ATPase that mediates Golgi uptake of cytosolic Ca(2+) and Mg(2+) and has a role in regulating Ca(2+) and Mn(2+) cellular content (PMID: 11741891). ATP2C1 is found in most tissues except colon, thymus, spleen, and leukocytes. It is expressed in keratinocytes (PMID: 15831496, 14632183). Defects in ATP2C1 cause Hailey-Hailey disease (HHD)(PMID: 10615129, 28035777).

Publications

Species	Application	Title
human	WB,IF	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
human	WB	Front Cell Dev Biol; A Negative Feedback Loop in Ultraviolet A-Induced Senescence in Human Dermal Fibroblasts Formed by SPCA1 and MAPK.; Authors - Hongfu Xie
human	WB	Clin Cosmet Investig Dermatol; The Pathogenic Mechanism of the ATP2C1 p.Ala109_Gln120del Mutation in Hailey-Hailey Disease; Authors - Peiyao Li
mouse	WB	BMC Pulm Med; Tetramethylpyrazine ameliorates endotoxin-induced acute lung injury by relieving Golgi stress via the Nrf2/HO-1 signaling pathway; Authors - Shaona Li
mouse	WB	Zhong Nan Da Xue Xue Bao Yi Xue Ban; Ultrashort wave alleviates oxygen-glucose deprivation/reoxygenation injury via up-regulation of SPCA1 expression in N2a cells; Authors - Jinling Tang
human	IF	PLoS One; Chemigenetic Ca2+ indicators report elevated Ca2+ levels in endothelial Weibel-Palade bodies; Authors - Julian Terglane

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous ATP2C1 both for WB and IF. For WB, besides the band at the expected molecular weight, it also gives a non specific band at ~ 150 kd.

• Applications: Western Blot, Immunofluorescence,