ATP2C1 Polyclonal antibody

ATP2C1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 13310-1-AP

Synonyms

ATP dependent Ca(2+) pump PMR1, ATP2C1, ATP2C1A, ATPase 2C1, HHD, hSPCA1, KIAA1347, PMR1, PMR1L, SPCA1

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Validation Data Gallery

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  WB analysis of HeLa using 13310-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 13310-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13310-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 13310-1-AP

  IP Result of anti-ATP2C1 (IP:13310-1-AP, 4ug; Detection:13310-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IF Staining of HeLa using 13310-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13310-1-AP (ATP2C1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, human brain tissue
• Positive IP detected in: mouse kidney tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

13310-1-AP targets ATP2C1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP2C1 fusion protein Ag4143
• Full Name: ATPase, Ca++ transporting, type 2C, member 1
• Calculated molecular weight: 919 aa, 101 kDa
• Observed molecular weight: 95-103 kDa
• GenBank accession number: BC028139
• Gene symbol: ATP2C1
• Gene ID (NCBI): 27032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; A Negative Feedback Loop in Ultraviolet A-Induced Senescence in Human Dermal Fibroblasts Formed by SPCA1 and MAPK.; Authors - Hongfu Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous ATP2C1 both for WB and IF. For WB, besides the band at the expected molecular weight, it also gives a non specific band at ~ 150 kd.

• Applications: Western Blot, Immunofluorescence,