ATP2C1 Polyclonal antibody

ATP2C1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13310-1-AP

Synonyms

ATP dependent Ca(2+) pump PMR1, ATP2C1, ATP2C1A, ATPase 2C1, HHD, hSPCA1, KIAA1347, PMR1, PMR1L, SPCA1



Tested Applications

Positive WB detected inA431 cells, human brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

13310-1-AP targets ATP2C1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP2C1 fusion protein Ag4143
Full Name ATPase, Ca++ transporting, type 2C, member 1
Calculated molecular weight 919 aa, 101 kDa
Observed molecular weight 95-103 kDa
GenBank accession numberBC028139
Gene symbol ATP2C1
Gene ID (NCBI) 27032
RRIDAB_10640808
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2C1, also known as hSPCA1, belongs to the family of P-type cation transport ATPases. ATP2C1 is a Golgi-localized ATPase that mediates Golgi uptake of cytosolic Ca(2+) and Mg(2+) and has a role in regulating Ca(2+) and Mn(2+) cellular content (PMID: 11741891). ATP2C1 is found in most tissues except colon, thymus, spleen, and leukocytes. It is expressed in keratinocytes (PMID: 15831496, 14632183). Defects in ATP2C1 cause Hailey-Hailey disease (HHD)(PMID: 10615129, 28035777).

Protocols

Product Specific Protocols
WB protocol for ATP2C1 antibody 13310-1-APDownload protocol
IHC protocol for ATP2C1 antibody 13310-1-APDownload protocol
IF protocol for ATP2C1 antibody 13310-1-APDownload protocol
IP protocol for ATP2C1 antibody 13310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

A Negative Feedback Loop in Ultraviolet A-Induced Senescence in Human Dermal Fibroblasts Formed by SPCA1 and MAPK.

Authors - Hongfu Xie
humanWB

Clin Cosmet Investig Dermatol

The Pathogenic Mechanism of the ATP2C1 p.Ala109_Gln120del Mutation in Hailey-Hailey Disease

Authors - Peiyao Li
mouseWB

BMC Pulm Med

Tetramethylpyrazine ameliorates endotoxin-induced acute lung injury by relieving Golgi stress via the Nrf2/HO-1 signaling pathway

Authors - Shaona Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous ATP2C1 both for WB and IF. For WB, besides the band at the expected molecular weight, it also gives a non specific band at ~ 150 kd.

  • Applications: Western Blot, Immunofluorescence,