ATP5D Polyclonal antibody

ATP5D Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14893-1-AP

Synonyms

ATP5D, F ATPase delta subunit

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Validation Data Gallery

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  WB analysis using 14893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14893-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, NIH/3T3 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

14893-1-AP targets ATP5D in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5D fusion protein Ag6682
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC002389
• Gene Symbol: ATP5D
• Gene ID (NCBI): 513
• RRID: AB_2274510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.

Publications

Species	Application	Title
mouse	WB	Theranostics; AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.; Authors - Liangjun Xia
human	WB	Int J Mol Sci; CA9 Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression.; Authors - Jiatong Xu
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He
human	WB	Am J Physiol Cell Physiol; 3-MST/H2S protects cerebral endothelial cells against OGD/R-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.; Authors - Fang Zhang
human	IF	J Neuropathol Exp Neurol; Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease.; Authors - Belén Ansoleaga
human	WB	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas