|Positive WB detected in||A549 cells, NIH/3T3 cells, HeLa cells, HepG2 cells|
|Positive IHC detected in||human liver cancer tissue, human lung cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:600|
|Sample-dependent, check data in validation data gallery|
14893-1-AP targets ATP5D in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP5D fusion protein Ag6682|
|Full Name||ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit|
|Calculated molecular weight||17 kDa|
|Observed molecular weight||15-17 kDa|
|GenBank accession number||BC002389|
|Gene ID (NCBI)||513|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.
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