ATP5D Polyclonal antibody

ATP5D Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14893-1-AP

Synonyms

ATP5D, F ATPase delta subunit



Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:600
Sample-dependent, check data in validation data gallery

Product Information

14893-1-AP targets ATP5D in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5D fusion protein Ag6682
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
Calculated molecular weight 17 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC002389
Gene symbol ATP5D
Gene ID (NCBI) 513
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.

Protocols

Product Specific Protocols
WB protocol for ATP5D antibody 14893-1-APDownload protocol
IHC protocol for ATP5D antibody 14893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.

Authors - Anaïs Aulas
humanWB

Int J Mol Sci

CA9 Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression.

Authors - Jiatong Xu
humanIF

J Neuropathol Exp Neurol

Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease.

Authors - Belén Ansoleaga
humanWB

Am J Physiol Cell Physiol

3-MST/H2S protects cerebral endothelial cells against OGD/R-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.

Authors - Fang Zhang