ATP5J Polyclonal antibody

ATP5J Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 14114-1-AP

Synonyms

ATP5, ATP5A, ATP5J, ATPase subunit F6, ATPM, CF6, F6

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Validation Data Gallery

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  WB analysis using 14114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14114-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14114-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14114-1-AP

  IP result of anti-ATP5J (IP:14114-1-AP, 4ug; Detection:14114-1-AP 1:300) with HEK-293 cells lysate 3680ug.

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  IHC staining of human osteosarcoma using 14114-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 14114-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HUVEC cells, mouse liver tissue, human heart tissue, SKOV-3 cells, mouse heart tissues, rat heart tissues
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human osteosarcoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

14114-1-AP targets ATP5J in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5J fusion protein Ag5263
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC066310
• Gene Symbol: ATP5J
• Gene ID (NCBI): 522
• RRID: AB_2062056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5J, also known as coupling factor 6 (CF6), is a soluble integral component of mitochondrial ATP synthase. Mitochondrial ATP synthase is a multi-subunit membrane-bound enzyme that catalyzes the synthesis of ATP by utilizing a proton electrochemical gradient. It consists of three domains, namely the extrinsic and intrinsic membrane domains (F1 and F0, respectively) joined by a stalk. CF6 is one of the subunits in the stalk and an essential component for energy transduction. Recently CF6 has also been reported to play a crucial role in the development of INS resistance and hypertension. CF6 is first synthesized as an immature form in the cytosol, then transported to the mitochondria by an import signal peptide and becomes an active form with the signal peptide cleaved. Western blot analysis of CF6 demonstrates a single band around 9 kDa to 12 kDa in various tissues including heart, liver, brain and HUVEC (human umbilical vein endothelial cells).

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.; Authors - Linyi Song
mouse	WB	Arch Pharm Res; Overexpressing microRNA-203 alleviates myocardial infarction via interacting with long non-coding RNA MIAT and mitochondrial coupling factor 6.; Authors - Fan Wang
rabbit	WB	J Proteomics; Congenital cataracts affect the retinal visual cycle and mitochondrial function: A multi-omics study of GJA8 knockout rabbits; Authors - Weijie Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

P (Verified Customer) (05-16-2024)

Good

• Applications: Western Blot
• Cell Tissue Type: Ac16

ATP5J Antibody Western Blot validation ( dilution) in Ac16 (Cat no:14114-1-AP)