Validation Data Gallery
|Positive WB detected in||A431 cells, human placenta tissue, mouse kidney tissue, Daudi cells, HeLa cells, HepG2 cells, K-562 cells, U-87 MG cells, mouse testis tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human pancreas tissue, human thyroid cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17115-1-AP targets ATP6V1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, zebrafish, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP6V1A fusion protein Ag10801|
|Full Name||ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A|
|Calculated molecular weight||617 aa, 68 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC013138|
|Gene ID (NCBI)||523|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.
Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.
Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.
The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.
Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency
Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.
Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.