ATP6V1A Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP6V1A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

31

Cat no : 17115-1-AP

Synonyms

ATP6A1, ATP6V1A, ATP6V1A1, HO68, V ATPase 69 kDa subunit, V ATPase subunit A, VA68, Vacuolar ATPase isoform VA68, Vma1, VPP2

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Validation Data Gallery

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  WB analysis using 17115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17115-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17115-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17115-1-AP

  IP Result of anti-ATP6V1A (IP:17115-1-AP, 4ug; Detection:17115-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human pancreas using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human thyroid cancer using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, human placenta tissue, mouse kidney tissue, Daudi cells, HeLa cells, HepG2 cells, K-562 cells, U-87 MG cells, mouse testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 10 publications below
• IP: See 2 publications below

Product Information

17115-1-AP targets ATP6V1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, zebrafish, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1A fusion protein Ag10801
• Full Name: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Calculated molecular weight: 617 aa, 68 kDa
• Observed molecular weight: 68 kDa
• GenBank accession number: BC013138
• Gene symbol: ATP6V1A
• Gene ID (NCBI): 523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Publications

Species	Application	Title
human	WB	Cell; Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.; Authors - Zharko Daniloski
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
human	WB	Nat Microbiol; The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.; Authors - Tomas Doyle
human	WB	Autophagy; Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency; Authors - Fangyang Wang
human,mouse	WB	Mol Psychiatry; Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.; Authors - A Pérez-Cañamás
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong