|Positive WB detected in||mouse kidney tissue, HeLa cells, human placenta tissue, K-562 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17115-1-AP targets ATP6V1A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP6V1A fusion protein Ag10801|
|Full Name||ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A|
|Calculated molecular weight||617 aa, 68 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC013138|
|Gene ID (NCBI)||523|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.
Mol Cell Proteomics
Spatiotemporal changes of the phagosomal proteome in dendritic cells in response to LPS stimulation.
Cell Death Dis
DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.
Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.
The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish.
J Cell Physiol
A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.
Cell Death Dis
Proton pump inhibitors protect mice from acute systemic inflammation and induce long-term cross-tolerance.