ATP6V1A Polyclonal antibody

ATP6V1A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 17115-1-AP

Synonyms

ATP6A1, ATP6V1A, ATP6V1A1, HO68, V ATPase 69 kDa subunit, V ATPase subunit A, VA68, Vacuolar ATPase isoform VA68, Vma1, VPP2



Tested Applications

Positive WB detected inmouse kidney tissue, A431 cells, HeLa cells, human placenta tissue, K-562 cells, Daudi cells, HepG2 cells, U-87 MG cells, mouse testis
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17115-1-AP targets ATP6V1A in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1A fusion protein Ag10801
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC013138
Gene symbol ATP6V1A
Gene ID (NCBI) 523
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Protocols

Product Specific Protocols
WB protocol for ATP6V1A antibody 17115-1-APDownload protocol
IHC protocol for ATP6V1A antibody 17115-1-APDownload protocol
IP protocol for ATP6V1A antibody 17115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Spatiotemporal changes of the phagosomal proteome in dendritic cells in response to LPS stimulation.

Authors - Anne-Marie Pauwels
mouseWB

Cell Death Dis

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Authors - Yan Cao
humanWB

Vet Res

Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.

Authors - Bingxin Chu
zebrafishIF

Dev Biol

The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish.

Authors - Jason J Gokey
ratWB,IF

J Cell Physiol

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Authors - Vishwanatha K Rao
zebrafishIF,WB

Dis Model Mech

Loss of vacuolar-type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromasts.

Authors - Peu Santra
  • KO Validated