ATPIF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATPIF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 12067-1-AP

Synonyms

IF(1), ATPIP, ATPI, ATPase inhibitory factor 1, ATP5IF1

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Validation Data Gallery

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  WB analysis of HeLa using 12067-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12067-1-AP (ATPIF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12067-1-AP

  IP result of anti-ATPIF1 (IP:12067-1-AP, 4ug; Detection:12067-1-AP 1:500) with HeLa cells lysate 3440ug.

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  IHC staining of human liver using 12067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12067-1-AP (ATPIF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12067-1-AP (ATPIF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12067-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPIF1 antibody (12067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

12067-1-AP targets ATPIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATPIF1 fusion protein Ag2704
• Full Name: ATPase inhibitory factor 1
• Calculated Molecular Weight: 106 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC009677
• Gene Symbol: ATPIF1
• Gene ID (NCBI): 93974
• RRID: AB_2063159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The H(+)-ATP synthase is a reversible engine of mitochondria that synthesizes or hydrolyzes ATP upon changes in cell physiology. ATP synthase dysfunction is involved in the onset and progression of diverse human pathologies. ATPIF1 gene encodes mitochondrial ATPase Inhibitory Factor 1 (IF1), also named ATPI, ATPIP or IP. Endogenous IF1 limits ATP depletion when the mitochondrial membrane potential falls below a threshold and the ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Mitochondrial content of IF1 controls the activity of oxidative phosphorylation mediating the shift of cancer cells to an enhanced aerobic glycolysis, thus supporting an oncogenic role of IF1 in cancer.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
	IF	Br J Pharmacol; The compound BTB06584 is an IF1 -dependent selective inhibitor of the mitochondrial F1 Fo-ATPase.; Authors - Fabrice Ivanes
human	IF,WB	Cell Biosci; ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells.; Authors - Kang Wang
	WB,IF	Lab Invest; ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis; Authors - Kailiang Zhang ; KD Validated
	WB,IF	Lab Invest; ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis.; Authors - Kailiang Zhang ; KD Validated
	IHC,IF	Front Physiol; F1F0-ATP Synthase Inhibitory Factor 1 in the Normal Pancreas and in Pancreatic Ductal Adenocarcinoma: Effects on Bioenergetics, Invasion and Proliferation.; Authors - Helen Tanton