Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12067-1-AP targets ATPIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2704 Product name: Recombinant human ATPIF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-106 aa of BC009677 Sequence: MAVTALAARTWLGVWGVRTMQARGFGSDQSENVDRGAGSIREAGGAFGKREQAEEERYFRAQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD Predict reactive species |
| Full Name | ATPase inhibitory factor 1 |
| Calculated Molecular Weight | 106 aa, 12 kDa |
| Observed Molecular Weight | 12 kDa |
| GenBank Accession Number | BC009677 |
| Gene Symbol | ATPIF1 |
| Gene ID (NCBI) | 93974 |
| RRID | AB_2063159 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UII2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The H(+)-ATP synthase is a reversible engine of mitochondria that synthesizes or hydrolyzes ATP upon changes in cell physiology. ATP synthase dysfunction is involved in the onset and progression of diverse human pathologies. ATPIF1 gene encodes mitochondrial ATPase Inhibitory Factor 1 (IF1), also named ATPI, ATPIP or IP. Endogenous IF1 limits ATP depletion when the mitochondrial membrane potential falls below a threshold and the ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Mitochondrial content of IF1 controls the activity of oxidative phosphorylation mediating the shift of cancer cells to an enhanced aerobic glycolysis, thus supporting an oncogenic role of IF1 in cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATPIF1 antibody 12067-1-AP | Download protocol |
| IHC protocol for ATPIF1 antibody 12067-1-AP | Download protocol |
| IP protocol for ATPIF1 antibody 12067-1-AP | Download protocol |
| WB protocol for ATPIF1 antibody 12067-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody targeting IF1 (ATP synthase inhibitory factor 1), catalog number 12067-1-AP, has 11 total citations. Journals include Nature Biotechnology, Nature Communications, British Journal of Pharmacology, Cell Biosciences, International Immunopharmacology, Laboratory Investigation, Frontiers in Physiology, Journal of Inherited Metabolic Disease, Frontiers in Oncology, and Digestive Diseases and Sciences. Research fields span expansion microscopy, mitochondrial biology, cardiac ischemia/reperfusion injury, pancreatic and colorectal cancer, islet β-cell function, and inherited metabolic disorders.
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Br J Pharmacol The compound BTB06584 is an IF1 -dependent selective inhibitor of the mitochondrial F1 Fo-ATPase. | ||
Cell Biosci ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells. | ||
Int Immunopharmacol GSK3β-mediated phosphorylation of IF1 at Ser27 destabilizes mitochondrial ATP synthase inhibitory factor 1 to aggravate cardiac ischemia/reperfusion injury. | ||
Lab Invest ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis
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