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Aurora A Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 10297-1-AP

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Con: 47 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Aurora A Antibody IF HeLa cells 10297-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Aurora A Antibody WB HEK-293 cells 10297-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10297-1-AP( Aurora A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

Aurora A Antibody IHC human breast cancer tissue 10297-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP( Aurora A antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Aurora A Antibody IHC human breast cancer tissue 10297-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP( Aurora A antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, SKOV-3 cells, SW 1990 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Aurora A fusion protein Ag0362
Full name:
aurora kinase A
Calculated molecular weight:
46 kDa
Observed molecular weight:
46 kDa
GenBank accession number:
BC002499
Gene ID (NCBI):
6790
Gene symbol
AURKA
Synonyms
AIK, ARK 1, ARK1, AURA, AURKA, Aurora A, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

Background

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).


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