Aurora A Polyclonal antibody

• Featured Product
• KD/KO Validated

Aurora A Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10297-1-AP

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, Aurora A, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

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Validation Data Gallery

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  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

10297-1-AP targets Aurora A in IHC, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aurora A fusion protein Ag0362
• Full Name: aurora kinase A
• Calculated molecular weight: 46 kDa
• Observed molecular weight: 46 kDa
• GenBank accession number: BC002499
• Gene symbol: AURKA
• Gene ID (NCBI): 6790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publications

Species	Application	Title
human	WB	Technol Cancer Res Treat; PMID: 31570091; Authors - Yan Li Yuan
human	WB	Biomed Res Int; AURKA Increase the Chemosensitivity of Colon Cancer Cells to Oxaliplatin by Inhibiting the TP53-Mediated DNA Damage Response Genes.; Authors - Baocong Shan ; KD Validated
human	WB	Acta Pharmacol Sin; Avasimibe exerts anticancer effects on human glioblastoma cells via inducing cell apoptosis and cell cycle arrest.; Authors - Jin-Yi Liu
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen
human	WB	J Exp Clin Cancer Res; 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.; Authors - Wan Li
human	WB	Eur J Med Chem; Design, synthesis, biological evaluation of 6-(2-amino-1H-benzo[d]imidazole-6-yl)quinazolin-4(3H)-one derivatives as novel anticancer agents with Aurora kinase inhibition.; Authors - Chengcheng Fan