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  • KD/KO Validated

Aurora A Polyclonal antibody

Aurora A Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10297-1-AP

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, Aurora A, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7



Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10297-1-AP targets Aurora A in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aurora A fusion protein Ag0362
Full Name aurora kinase A
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002499
Gene symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Protocols

Product Specific Protocols
IHC protocol for Aurora A antibody 10297-1-APDownload protocol
IF protocol for Aurora A antibody 10297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Technol Cancer Res Treat

PMID: 31570091

Authors - Yan Li Yuan
humanWB

Biomed Res Int

AURKA Increase the Chemosensitivity of Colon Cancer Cells to Oxaliplatin by Inhibiting the TP53-Mediated DNA Damage Response Genes.

Authors - Baocong Shan
  • KD Validated
humanWB

Acta Pharmacol Sin

Avasimibe exerts anticancer effects on human glioblastoma cells via inducing cell apoptosis and cell cycle arrest.

Authors - Jin-Yi Liu
humanWB

Mol Pharm

Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.

Authors - Xiaoman Chen
humanWB

J Exp Clin Cancer Res

3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.

Authors - Wan Li
humanWB

Oncotarget

Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.

Authors - Yanwei Liu