AURKA Polyclonal antibody

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Aurora A antibody (10297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10297-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Aurora A (10297-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

AURKA Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10297-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Aurora A, aurora kinase A, AURK, AURA, ARK-1

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

10297-1-AP targets AURKA in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AURKA fusion protein Ag0362
• Full Name: aurora kinase A
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002499
• Gene Symbol: AURKA
• Gene ID (NCBI): 6790
• RRID: AB_2061337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14965
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publications

Species	Application	Title
human		Acta Pharm Sin B; Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.; Authors - Xiangjin Zheng
mouse	IF	Nat Commun; CPEB1 directs muscle stem cell activation by reprogramming the translational landscape.; Authors - Wenshu Zeng
human		Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human		Eur J Med Chem; Design, synthesis, biological evaluation of 6-(2-amino-1H-benzo[d]imidazole-6-yl)quinazolin-4(3H)-one derivatives as novel anticancer agents with Aurora kinase inhibition.; Authors - Chengcheng Fan
human		Front Immunol; The ALDH Family Contributes to Immunocyte Infiltration, Proliferation and Epithelial-Mesenchymal Transformation in Glioma.; Authors - Zeyu Wang
human		J Exp Clin Cancer Res; 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.; Authors - Wan Li