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AURKA Monoclonal antibody
AURKA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IHC, IF/ICC, CoIP, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
1F4B10
Cat no : 66757-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human testis tissue, HeLa cells, NCCIT cells, rat testis tissue, mouse testis tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 13 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
Product Information
66757-1-Ig targets AURKA in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AURKA fusion protein Ag20096 |
Full Name | aurora kinase A |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 46-52 kDa |
GenBank Accession Number | BC002499 |
Gene Symbol | AURKA |
Gene ID (NCBI) | 6790 |
RRID | AB_2882103 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).
Protocols
Product Specific Protocols | |
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WB protocol for AURKA antibody 66757-1-Ig | Download protocol |
IHC protocol for AURKA antibody 66757-1-Ig | Download protocol |
IF protocol for AURKA antibody 66757-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Oncol PUF60/AURKA Axis Contributes to Tumor Progression and Malignant Phenotypes in Bladder Cancer. | ||
Front Oncol Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression. | ||
Front Oncol Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities
| ||
Biochem Pharmacol Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA. | ||
Carcinogenesis ARID3A promotes the development of colorectal cancer by upregulating AURKA. | ||
Biochim Biophys Acta Mol Basis Dis Blocking AURKA with MK-5108 attenuates renal fibrosis in chronic kidney disease. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah-Eve (Verified Customer) (06-02-2023) | Works well, WB dilution 1:2000
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