Tested Applications
| Positive WB detected in | human testis tissue, HeLa cells, NCCIT cells, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66757-1-Ig targets AURKA in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20096 Product name: Recombinant human AURKA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 108-337 aa of BC002499 Sequence: NNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQET Predict reactive species |
| Full Name | aurora kinase A |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46-52 kDa |
| GenBank Accession Number | BC002499 |
| Gene Symbol | AURKA |
| Gene ID (NCBI) | 6790 |
| RRID | AB_2882103 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14965 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AURKA antibody 66757-1-Ig | Download protocol |
| IHC protocol for AURKA antibody 66757-1-Ig | Download protocol |
| WB protocol for AURKA antibody 66757-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AURKA Monoclonal antibody (Cat# 66757-1-Ig) has 43 citations. Publications appear in journals including Acta Pharm Sin B, Cell Reports in Medicine, Cell Death & Disease, Journal for ImmunoTherapy of Cancer, Current Biology, FASEB Journal, Frontiers in Oncology, Endocrinology, and PLoS One. Research fields span oncology (breast, liver, bladder, thyroid, prostate, melanoma, colorectal cancers), cell cycle and mitosis, fibrosis, inflammation, immunology, epigenetics, and reproductive biology, with applications mainly in WB, IF, IHC, IP, and ChIP.
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B BRD4 regulates m6A of ESPL1 mRNA via interaction with ALKBH5 to modulate breast cancer progression | ||
Cell Rep Med Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies | ||
Cell Death Dis AURKA suppresses NCOA4-mediated ferritinophagy to enhance sorafenib resistance in hepatocellular carcinoma.
| ||
Cell Death Dis Pre-rRNAs control mitosis by maintaining chromosomal segregation through protecting SMC2 from AURKA-mediated phosphorylation. | ||
J Immunother Cancer Adenovirus vaccine targeting kinases induces potent antitumor immunity in solid tumors | ||
Cell Commun Signal SENP5 enhances antiviral innate immunity by promoting AURKA-dependent STAT2 phosphorylation. |
Reviews
What customers say
The product received a 5-star rating from a user who tested it on human pulmonary artery smooth muscle cells for Western Blot at a 1:2000 dilution. The reviewer reported that it "works well," indicating satisfactory performance and signal quality for their application. Overall, the feedback is positive, confirming reliable results in WB experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah-Eve (Verified Customer) (06-02-2023) | Works well, WB dilution 1:2000
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