• Featured Product
  • KD/KO Validated

AURKA Monoclonal antibody

AURKA Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG3


Human, Mouse, Rat and More (2)







Cat no : 66757-1-Ig


AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

Tested Applications

Positive WB detected inhuman testis tissue, HeLa cells, NCCIT cells, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66757-1-Ig targets AURKA in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen AURKA fusion protein Ag20096
Full Name aurora kinase A
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).


Product Specific Protocols
WB protocol for AURKA antibody 66757-1-IgDownload protocol
IHC protocol for AURKA antibody 66757-1-IgDownload protocol
IF protocol for AURKA antibody 66757-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Oncol

PUF60/AURKA Axis Contributes to Tumor Progression and Malignant Phenotypes in Bladder Cancer.

Authors - Qian Long

Front Oncol

Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression.

Authors - Bo Liang

Front Oncol

Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities

Authors - Jing Hang
  • KD Validated

Biochem Pharmacol

Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA.

Authors - Xiaojiang Liu


ARID3A promotes the development of colorectal cancer by upregulating AURKA.

Authors - Jing Tang

Biochim Biophys Acta Mol Basis Dis

Blocking AURKA with MK-5108 attenuates renal fibrosis in chronic kidney disease.

Authors - Mingzhu Jiang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sarah-Eve (Verified Customer) (06-02-2023)

Works well, WB dilution 1:2000

  • Applications: Western Blot
  • Cell Tissue Type: Human pulmonary artery smooth muscle cells