AXIN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AXIN2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

38

Reviews (2)

Cat no : 20540-1-AP

Synonyms

AXIL, axin 2, Axin like protein, AXIN2, Axis inhibition protein 2, Conductin

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Validation Data Gallery

• 

  WB analysis using 20540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20540-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 20540-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 20540-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 20540-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20540-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, MDA-MB-453s cells, BxPC-3 cells, HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 37 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

20540-1-AP targets AXIN2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AXIN2 fusion protein Ag14574
• Full Name: axin 2
• Calculated molecular weight: 843 aa, 94 kDa
• Observed molecular weight: 94-100 kDa
• GenBank accession number: BC006295
• Gene symbol: AXIN2
• Gene ID (NCBI): 8313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutaion is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibdy raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Adv Healthc Mater; Ultrathin 2D Titanium Carbide MXene (Ti3 C2 Tx ) Nanoflakes Activate WNT/HIF-1α-Mediated Metabolism Reprogramming for Periodontal Regeneration.; Authors - Di Cui
human	WB	Autophagy; TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.; Authors - Cefan Zhou
human	WB	Theranostics; Targeted inhibition of KDM6 histone demethylases eradicates tumor-initiating cells via enhancer reprogramming in colorectal cancer.; Authors - Junbao Zhang
human	WB	Oncogene; Identification of a novel Calpain-2-SRC feed-back loop as necessity for β-Catenin accumulation and signaling activation in hepatocellular carcinoma.; Authors - Xiao-Lu Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aurélie (Verified Customer) (10-06-2023)

This antibody gives a specific band at 100 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: RKO

AXIN2 Antibody Western Blot validation (1/2000 dilution) in RKO (Cat no:20540-1-AP)

FH

Guannan (Verified Customer) (01-31-2019)

This antibody gives me a band at 95 kd, but also a lot of nonspecific bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7

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