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AXIN2 Polyclonal antibody

AXIN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20540-1-AP


AXIL, axin 2, Axin like protein, AXIN2, Axis inhibition protein 2, Conductin

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, MDA-MB-453s cells, BxPC-3 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20540-1-AP targets AXIN2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AXIN2 fusion protein Ag14574
Full Name axin 2
Calculated Molecular Weight 843 aa, 94 kDa
Observed Molecular Weight 94-100 kDa
GenBank Accession NumberBC006295
Gene Symbol AXIN2
Gene ID (NCBI) 8313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutaion is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibdy raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).


Product Specific Protocols
WB protocol for AXIN2 antibody 20540-1-APDownload protocol
IHC protocol for AXIN2 antibody 20540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Aurélie (Verified Customer) (10-06-2023)

This antibody gives a specific band at 100 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: RKO
AXIN2 Antibody Western Blot validation (1/2000 dilution) in RKO (Cat no:20540-1-AP)

Guannan (Verified Customer) (01-31-2019)

This antibody gives me a band at 95 kd, but also a lot of nonspecific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF7