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  • KD/KO Validated

AXIN2 Antibody

AXIN2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20540-1-AP

Synonyms

AXIL, axin 2, Axin like protein, AXIN2, Axis inhibition protein 2, Conductin



Tested Applications

Positive WB detected inJurkat cells, BxPC-3 cells, MDA-MB-453s cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 20540-1-AP immunogen is Fusion Protein AXIN2 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AXIN2 fusion protein Ag14574
Full Name axin 2
Calculated molecular weight 843aa,94 kDa
Observed molecular weight 93-100 kDa
GenBank accession number BC006295
Gene symbol AXIN2
Gene ID (NCBI) 8313
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutaion is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibdy raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).

Protocols

Product Specific Protocols
WB protocol for AXIN2 antibody 20540-1-APDownload protocol
IHC protocol for AXIN2 antibody 20540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Res

Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress.

Authors - Yue Zong
humanWB

Cell Death Dis

PMID: 31186404

Authors - Jingsheng Yuan
humanWB

Cell Death Dis

circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.

Authors - Zhifei Ma
mouseWB

BMC Cancer

PMID: 31931755

Authors - Xia Liao
humanWB

Onco Targets Ther

TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling.

Authors - Xiumei Zhang
  • KD Validated
humanWB

Exp Cell Res

Secreted AGR2 promotes invasion of colorectal cancer cells via Wnt11-mediated non-canonical Wnt signaling.

Authors - Shaobo Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guannan (Verified Customer) (01-31-2019)

This antibody gives me a band at 95 kd, but also a lot of nonspecific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF7
No image submitted with review