AXIN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AXIN2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (1)

Cat no : 20540-1-AP

Synonyms

AXIL, axin 2, Axin like protein, AXIN2, Axis inhibition protein 2, Conductin

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 20540-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 20540-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 20540-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 20540-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20540-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells, BxPC-3 cells, MDA-MB-453s cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20540-1-AP is AXIN2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AXIN2 fusion protein Ag14574
• Full Name: axin 2
• Calculated molecular weight: 843 aa, 94 kDa
• Observed molecular weight: 93-100 kDa
• GenBank accession number: BC006295
• Gene symbol: AXIN2
• Gene ID (NCBI): 8313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutaion is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibdy raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).

Publications

Species	Application	Title
	WB	Cell Res; Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress.; Authors - Yue Zong
human	WB	Cell Death Dis; PMID: 31186404; Authors - Jingsheng Yuan
human	WB	Cell Death Dis; circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.; Authors - Zhifei Ma
human	WB	Med Sci Monit; Long Non-Coding RNA THOR Enhances the Stem Cell-Like Traits of Triple-Negative Breast Cancer Cells Through Activating β-Catenin Signaling.; Authors - Binbin Wang
mouse	WB	Mol Biol (Mosk); [PARP10 Influences the Proliferation of Colorectal Carcinoma Cells, a Preliminary Study].; Authors - C F Wu
human	WB	Adv Healthc Mater; Ultrathin 2D Titanium Carbide MXene (Ti3 C2 Tx ) Nanoflakes Activate WNT/HIF-1α-Mediated Metabolism Reprogramming for Periodontal Regeneration.; Authors - Di Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Guannan (Verified Customer) (01-31-2019)

This antibody gives me a band at 95 kd, but also a lot of nonspecific bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7

Related Products

AXIN1 Polyclonal antibody

16541-1-AP

Beta Catenin Polyclonal antibody

51067-2-AP

TCF4/TCF7L2 Polyclonal antibody

13838-1-AP

APC Polyclonal antibody

19782-1-AP

GSK3B Polyclonal antibody

22104-1-AP

E-cadherin Polyclonal antibody

20874-1-AP