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AXIN2 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 20540-1-AP

1 customer review

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Con: 70 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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AXIN2 Antibody IHC human lung cancer tissue 20540-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

AXIN2 Antibody IHC human lung cancer tissue 20540-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

AXIN2 Antibody WB Jurkat cells 20540-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 20540-1-AP( AXIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, BxPC-3 cells, MDA-MB-453s cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AXIN2 fusion protein Ag14574
Full name:
axin 2
Calculated molecular weight:
843aa,94 kDa
Observed molecular weight:
93-100 kDa
GenBank accession number:
BC006295
Gene ID (NCBI):
8313
Gene symbol
AXIN2
Synonyms
AXIL, axin 2, Axin like protein, AXIN2, Axis inhibition protein 2, Conductin

Background

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutaion is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibdy raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

AXIN2 Rabbit Polyclonal antibody

Guannan (Verified Customer), 01-31-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
MCF7
Lot Number
This antibody gives me a band at 95 kd, but also a lot of nonspecific bands.
Proteintech Response
Thank you for your feedback. We will re-examine this product on your sample type to see if we get the same results.

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