Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, HepG2 cells, human liver tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13196-1-AP targets AXL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3898 Product name: Recombinant human AXL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 545-894 aa of BC032229 Sequence: GEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRRGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA Predict reactive species |
| Full Name | AXL receptor tyrosine kinase |
| Calculated Molecular Weight | 894 aa, 98 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC032229 |
| Gene Symbol | AXL |
| Gene ID (NCBI) | 558 |
| RRID | AB_10642006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for AXL antibody 13196-1-AP | Download protocol |
| WB protocol for AXL antibody 13196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AXL Polyclonal antibody (Cat# 13196-1-AP) has 51 citations in journals including Nature Metabolism, ACS Nano, Theranostics, Advanced Science, Cell Death & Disease, Cancer Letters, Clinical Cancer Research, Cell Reports, Frontiers in Immunology, Transplantation, iScience, and PLoS Pathogens. Associated research fields include oncology (lung, breast, pancreatic, melanoma, glioma, renal, and bladder cancers), neurology (stroke, Alzheimer's), immunology, fibrosis, virology (Zika, SARS-CoV-2), cardiovascular disease, and glucose metabolism.
| Species | Application | Title |
|---|---|---|
ACS Nano Personalized Pathogenic Nanoplastic Coronas Orchestrate Efferocytosis-Driven Immune Evasion in Lung Adenocarcinoma. | ||
Theranostics CD146(+) pericyte-like lung cancer brain metastatic stem cells promote tumor angiogenesis through dual regulatory effects on the VEGF/VEGFR axis. | ||
Adv Sci (Weinh) EGFR Targeted Liposomal PROTAC Assisted With Epigenetic Regulation as an Efficient Strategy for Osimertinib-Resistant Lung Cancer Therapy | ||
Cell Death Dis Identification of PRRG1 as a possible molecular target of pancreatic cancer. | ||
Cell Death Dis Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop
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Reviews
What customers say
One reviewer (Marta M.) rated this antibody 4/5 stars after using it at a 1:2000 dilution for Western Blot on A375 melanoma cell lysate. She reported that the antibody performed well with no non-specific bands detected, indicating good specificity in her application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marta (Verified Customer) (05-30-2022) | The antibody works well. Non-specific bands are not detected.
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