AbFlex Histone H3K9ac antibody (rAb)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, ELISA, ICC, IF, WB
Cat No : 91103,91104 91103
Synonyms
Validation Data Gallery
Product Information
Tested Applications |
ChIP, ChIP-Seq, ELISA, ICC, IF, WB
Applications Validated by Active Motif: ChIP-Seq: 4 ug each ICC/IF: 1 - 2 ug/ml WB*: 0.2 - 2 ug/ml Bead-based ELISA: 1.5 - 40 ug/ml CUT&Tag: 1 ug per 50 ul reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental antibody clone, see Catalog No. 61251. |
Tested Reactivity | Human, Wide Range Predicted |
Host / Isotype | Mouse / IgG2a |
Class | Recombinant |
Type | Antibody |
Modification | Acetylated |
Immunogen | This antibody was raised against a peptide containing acetyl-lysine 9 of human Histone H3. |
Full Name | AbFlex Histone H3K9ac antibody (rAb) |
Synonyms | Histone H3, Histone acetylation, transcriptional activation, chromatin, nucleosome, antibody, antibodies, polyclonal, H3 AcK9, H3K9Ac, chromatin immunoprecipitation, chip, recombinant, rAb, rec Ab, AbFlex |
Molecular weight | 17 kDa |
GenBank accession number | NP_003522 |
RRID | AB_2793775 |
Purification Method | Ni-NTA |
Buffer | Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K9ac antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions.Acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes.