AbFlex N6-Methyladenosine (m6A) antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Not Species Specific

Applications

IP, Dot Blot

Cat No : 91261,91262

Synonyms

N6-Metheyladenosine, m6A, methyladenosine, AbFlex, recombinant antibody

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Validation Data Gallery

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  AbFlex N6-Methyladenosine (m6A) antibody tested by Dot blot. 3.3 ng of single-stranded DNA oligonucleotides were spotted on to a positively charged nylon membrane and blotted with AbFlex N-6-methyladenosine recombinant antibody (1 ug/ml dilution). 1. unmethylated sequence 1 2. single 6-methyl-adenosine in middle of sequence 1 3. unmethylated sequence 2 4. unmethylated sequence 3 5. single 1-methyl-adenosine in middle of sequence 3

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  AbFlex N6-Methyladenosine (m6A) antibody tested by RNA immunoprecipitation. (A) In vitro RNA transcripts were generated containing either 0 or 10% of the m6A modified base or the A unmodified base and were spiked into 10 ug of total RNA. IP was performed using 10 ug Abflex N6-Methyladenosine antibody followed by Real Time-qPCR. (B) IP was performed using 5 ug Abflex N6-Methyladenosine antibody and 30 ug of total RNA (fragmented to 200 bp) from human hematopoetic stem cells followed by Real Time-qPCR on known methylated RNA. Data was provided courtesy of Dr. Alexandra Patmanidi in Prof. Dr. Achim Leutz’s lab, Max-Delbrück-Centrum für Molekulare Medizin (MDC).

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Product Information

• Tested Applications: IP, Dot Blot

  Applications Validated by Active Motif: DB IP: 5 - 10 ug per IP

• Tested Reactivity: Human, Mouse, Not Species Specific
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Modification: Methylated
• Immunogen: This antibody was raised against N6-methyladenosine conjugated to BSA.
• Full Name: AbFlex N6-Methyladenosine (m6A) antibody (rAb)
• Synonyms: N6-Metheyladenosine, m6A, methyladenosine, AbFlex, recombinant antibody
• GenBank accession number: N/A
• Purification Method: Protein A Chromatography
• Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex N6-Methyladenosine (m6A) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. N6-Methyladenosine (m6A) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.