ChromoTek Actin-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.

Specificity

Actin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagRFP

Cat no : acr

Synonyms

Actin Chromobody, Chromobody



Product Information

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.

DescriptionThe Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.

• Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time

• Target and trace microfilaments in your cell line of interest

• Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility

• Actin-Chromobody has no notable effect cell viability or migration

ApplicationsLive cell imaging
Visualizing actin dynamics
Actin as biomarker or control
SpecificityActin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
ReporterTagRFP
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
Nuclear localization signal (NLS)No
SequenceWith the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nat Methods

Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins.

Authors - Daseuli Yu

Nat Methods

Actin chromobody imaging reveals sub-organellar actin dynamics.

Authors - Cara R Schiavon

Nat Commun

Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases.

Authors - Vishnu Raman
IF

Nat Commun

tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis.

Authors - Aaron M Savage

EMBO J

F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes.

Authors - Bas van Bommel

Elife

Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation.

Authors - Javier Periz