ChromoTek Actin-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagRFP

Epitope

Actin

Publications

7

Cat no : acr

Synonyms

Actin Chromobody, Chromobody

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Validation Data Gallery

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  Nuclear Actin-Chromobody: NIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody (TagGFP2, green) and the Lamin-Chromobody (TagRFP, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum. Courtesy of Matthias Plessner.

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Product Information

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.

• Applications: Live cell imaging
• Specificity: Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Reporter: TagRFP
• Codon usage: Mammalian
• Selection: Kan/Neo
• Encoded Nanobody/ VHH: Monoclonal anti-Actin single domain antibody (sdAb) fragment
• Sequence: With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to nuclear Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Storage: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
IF	J Cell Sci; The interaction between CASK and the tumour suppressor Dlg1 regulates mitotic spindle orientation in mammalian epithelia.; Authors - Andrew P Porter
	Int Rev Cell Mol Biol; Development and Application of Functionalized Protein Binders in Multicellular Organisms.; Authors - D Bieli
	Elife; Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation.; Authors - Javier Periz
	EMBO J; F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes.; Authors - Bas van Bommel
IF	Nat Commun; tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis.; Authors - Aaron M Savage
	Nat Methods; Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins.; Authors - Daseuli Yu