ChromoTek Actin-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagRFP

Epitope

Actin

Cat no : acr

Synonyms



Product Information

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.

Applications Live cell imaging
SpecificityActin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagRFP
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-Actin single domain antibody (sdAb) fragment
SequenceWith the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to nuclear Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle
IF

J Cell Sci

The interaction between CASK and the tumour suppressor Dlg1 regulates mitotic spindle orientation in mammalian epithelia.

Authors - Andrew P Porter

Int Rev Cell Mol Biol

Development and Application of Functionalized Protein Binders in Multicellular Organisms.

Authors - D Bieli

Elife

Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation.

Authors - Javier Periz

EMBO J

F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes.

Authors - Bas van Bommel
IF

Nat Commun

tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis.

Authors - Aaron M Savage

Nat Methods

Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins.

Authors - Daseuli Yu