ChromoTek Actin-Chromobody® plasmid (TagRFP)
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
TagRFP
Epitope
Actin
Cat no : acr
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.
Applications | Live cell imaging |
Specificity | Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii |
Vector type | Mammalian expression vector |
Promoter | Constitutive CMV IE |
Reporter | TagRFP |
Codon usage | Mammalian |
Selection | Kan/Neo |
Encoded Nanobody/ VHH | Monoclonal anti-Actin single domain antibody (sdAb) fragment |
Sequence | With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to nuclear Actin fused to TagRFP or TagGFP2, as well as the full vector sequence. |
Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED |
Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
Storage | Shipped at ambient temperature. Store at -20 °C |
Documentation
Brochure |
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Product brochure (PDF) |
Datasheet |
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Datasheet Actin-Chromobody® TagGFP plasmid (PDF) |
Datasheet Actin-Chromobody® TagRFP plasmid (PDF) |
Datasheet Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
SDS |
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SDS Actin-Chromobody® TagGFP plasmid (PDF) |
SDS Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
Publications
Application | Title |
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J Cell Sci The interaction between CASK and the tumour suppressor Dlg1 regulates mitotic spindle orientation in mammalian epithelia. | |
Int Rev Cell Mol Biol Development and Application of Functionalized Protein Binders in Multicellular Organisms. | |
Elife Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation. | |
EMBO J F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes. | |
Nat Commun tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis. | |
Nat Methods Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. |