Cat no : acr
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.
|Description||The Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.
• Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time
• Target and trace microfilaments in your cell line of interest
• Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility
• Actin-Chromobody has no notable effect cell viability or migration
|Applications||Live cell imaging|
Visualizing actin dynamics
Actin as biomarker or control
|Specificity||Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii|
|Vector type||Mammalian expression vector|
|Promoter||Constitutive CMV IE|
|Nuclear localization signal (NLS)||No|
|Sequence||With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED|
|Storage Condition||Shipped at ambient temperature. Store at -20 °C|
|SDS Nuclear Actin-Chromobody® TagGFP plasmid (PDF)|
|SDS Actin-Chromobody® TagGFP plasmid (PDF)|
|Datasheet Actin-Chromobody® TagRFP plasmid (PDF)|
|Datasheet Actin-Chromobody® TagGFP plasmid (PDF)|
|Datasheet Nuclear Actin-Chromobody® TagGFP plasmid (PDF)|
|Chromotek nanobodies brochure (PDF)|
Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins.
Actin chromobody imaging reveals sub-organellar actin dynamics.
Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases.
tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis.
F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes.
Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation.