Aiolos/IKZF3 Recombinant antibody

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  WB analysis using 85752-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85752-2-RR (IKZF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Aiolos/IKZF3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85752-2-RR

Clone No.243113C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

IKZF3, AIO, AIOLOS, IKZF 3, Zinc finger protein Aiolos

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Tested Applications

• Positive WB detected in: mouse spleen tissue, rat spleen tissue, mouse thymus tissue, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85752-2-RR targets Aiolos/IKZF3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Aiolos/IKZF3 fusion protein Ag5537
• Full Name: IKAROS family zinc finger 3 (Aiolos)
• Calculated Molecular Weight: 509 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC032707
• Gene Symbol: IKZF3
• Gene ID (NCBI): 22806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKT9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.