APP/Beta Amyloid Polyclonal antibody

APP/Beta Amyloid Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

41

Reviews (1)

Cat no : 25524-1-AP

Synonyms

ABETA, ABPP, amyloid beta 1-42, Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, Aβ1-42, beta Amyloid

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Validation Data Gallery

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  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 25524-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 25524-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25524-1-AP (beta Amyloid (C-Terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, 3T3-L1 cells, HeLa cells, mouse brain tissue, rat brain tissue, C6 cells
• Positive IHC detected in: human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 9 publications below
• IF: See 11 publications below

Product Information

The immunogen of 25524-1-AP is APP/Beta Amyloid Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APP/Beta Amyloid fusion protein Ag22408
• Full Name: amyloid beta (A4) precursor protein
• Observed molecular weight: 100 kDa
• GenBank accession number: BC065529
• Gene symbol: APP
• Gene ID (NCBI): 351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.

Publications

Species	Application	Title
rat	WB	Neurosci Bull; PMID: 30632006; Authors - Yao Zhang
human,rat	WB	Neurotherapeutics; Direct Activation of Protein Phosphatase 2A (PP2A) by Tricyclic Sulfonamides Ameliorates Alzheimer's Disease Pathogenesis in Cell and Animal Models.; Authors - Hui Wei
mouse	IF	Aging (Albany NY); Functional genomics study of protein inhibitor of activated STAT1 in mouse hippocampal neuronal cells revealed by RNA sequencing.; Authors - Kan He
mouse	if	Acta Pharmacol Sin; Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function.; Authors - Xue Wang
rat	WB	Evid Based Complement Alternat Med; Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.; Authors - Rong Fan
mouse	WB	Int J Neurosci; Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice.; Authors - Yayun Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects APP in the brain of 12 months C57BL/6 mice.

• Applications: Western Blot,