APP/Beta Amyloid Polyclonal antibody

APP/Beta Amyloid Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

48

Reviews (2)

Cat no : 25524-1-AP

Synonyms

ABETA, ABPP, amyloid beta 1-42, Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, Aβ1-42, beta Amyloid

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Validation Data Gallery

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  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP/Beta Amyloid antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 25524-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25524-1-AP (beta Amyloid (C-Terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, mouse brain tissue, rat brain tissue, C6 cells
• Positive IHC detected in: human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 35 publications below
• IHC: See 11 publications below
• IF: See 11 publications below

Product Information

25524-1-AP targets APP/Beta Amyloid in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APP/Beta Amyloid fusion protein Ag22408
• Full Name: amyloid beta (A4) precursor protein
• Observed molecular weight: 100 kDa
• GenBank accession number: BC065529
• Gene symbol: APP
• Gene ID (NCBI): 351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.

Publications

Species	Application	Title
mouse	IF	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard
human	WB	Small; Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.; Authors - Qin Ouyang
mouse	IHC	Redox Biol; Cognitive enhancement and neuroprotective effects of OABL, a sesquiterpene lactone in 5xFAD Alzheimer's disease mice model.; Authors - Jiang-Jiang Tang
mouse,rat	WB,IF	Free Radic Biol Med; The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro; Authors - Jie Ai
human	IHC	Cancer Lett; LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.; Authors - Yawen Ma
mouse	WB, IHC	Sci Total Environ; The effects of antimony on Alzheimer's disease-like pathological changes in mice brain.; Authors - Shenya Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jorge (Verified Customer) (03-28-2023)

Test to evaluate the efficacy of the antibody. Tested on SH-SY5Y APPswe cells differentiated with 10 uM retic acid for 5 days. DAPT treatment 10 uM for 24h. Cells lysed with RIPA buffer. Loading different protein concentrations (ug). Tricine SDS-PAGE 16% acrylamide gel. Transfered on PVDF membrane. Blocking 5% milk. Dilution 1:4000 in TBS-Tw 0.05%.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Cell line SHSY5Y APPswe

APP/Beta Amyloid Antibody Western Blot validation (1:4000 dilution) in Cell line SHSY5Y APPswe (Cat no:25524-1-AP)

FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects APP in the brain of 12 months C57BL/6 mice.

• Applications: Western Blot,