APP/Beta Amyloid Polyclonal antibody

APP/Beta Amyloid Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 25524-1-AP


ABETA, ABPP, amyloid beta 1-42, Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, Aβ1-42, beta Amyloid

Tested Applications

Positive WB detected inSH-SY5Y cells, 3T3-L1 cells, HeLa cells, mouse brain tissue, rat brain tissue, C6 cells
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25524-1-AP is APP/Beta Amyloid Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APP/Beta Amyloid fusion protein Ag22408
Full Name amyloid beta (A4) precursor protein
Observed molecular weight 100 kDa
GenBank accession numberBC065529
Gene symbol APP
Gene ID (NCBI) 351
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.


Product Specific Protocols
WB protocol for APP/Beta Amyloid antibody 25524-1-APDownload protocol
IHC protocol for APP/Beta Amyloid antibody 25524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Neurosci Bull

PMID: 30632006

Authors - Yao Zhang


Direct Activation of Protein Phosphatase 2A (PP2A) by Tricyclic Sulfonamides Ameliorates Alzheimer's Disease Pathogenesis in Cell and Animal Models.

Authors - Hui Wei

Aging (Albany NY)

Functional genomics study of protein inhibitor of activated STAT1 in mouse hippocampal neuronal cells revealed by RNA sequencing.

Authors - Kan He

Acta Pharmacol Sin

Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function.

Authors - Xue Wang

Evid Based Complement Alternat Med

Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.

Authors - Rong Fan

Int J Neurosci

Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice.

Authors - Yayun Du


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


JIMMY (Verified Customer) (04-15-2019)

This antibody detects APP in the brain of 12 months C57BL/6 mice.

  • Applications: Western Blot,