Annexin A13 Polyclonal antibody

• 

  WB analysis using 25153-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 25153-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 25153-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 25153-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 40x lens).

Annexin A13 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25153-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ANXA13, Annexin 13, Annexin XIII, Annexin-13, ANX13

---

Tested Applications

• Positive WB detected in: mouse small intestine tissue, rat small intestine tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25153-1-AP targets Annexin A13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin A13 fusion protein Ag18581
• Full Name: annexin A13
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC125158
• Gene Symbol: Annexin A13
• Gene ID (NCBI): 312
• RRID: AB_2879928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27216
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A13 (ANXA13) is believed to be the original founder gene of the 12-member vertebrate annexin A family, and it has acquired an intestine-specific expression associated with a highly differentiated intracellular transport function (PMID: 11961095). ANXA13, which is highly expressed in the digestive system and believed to be the original founder gene of the annexin A family (PMID: 28423508). Limited evidence suggests that ANXA13 may play a role in apoptotic cell-mediated immunosuppression, hypertension, and cell death recognition (PMID: 28423508). ANXA13 is highly expressed in the human colon adenocarcinoma cell line HT29.

Publications

Species	Application	Title
human	WB	J Med Chem; Discovery of a Novel 1,4-Benzodiazepine Derivative as a Highly Selective ANXA3 Degrader for the Treatment of Triple-Negative Breast Cancer; Authors - Kunlin Liu