AuthentiKine™ Human IL-17A ELISA Kit

Sensitivity

0.36 pg/ml

Range

31.25-2000 pg/mL

Reactivity

Human

Cat no : KE00203

Synonyms

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL17A, IL-17A, interleukin 17A



Product Information

KE00203 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-17A ELISA kit is to be used to detect and quantify protein levels of endogenous IL-17A. The assay recognizes human IL-17A. An antibody specific for IL-17A has been pre-coated onto the microwells. The IL-17A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-17A is added to detect the captured IL-17A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IL-17A ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.36 pg/ml
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Cell culture supernatants 102%90%-116%
Human Plasma90%74%-107%

IntraAssay

Samplenmean ( pg/ml)SDCV%
120543.213.42.5
220129.45.54.2
32030.11.96.4

InterAssay

Samplenmean ( pg/ml)SDCV%
124555.231.45.7
224141.88.45.9
324282.59.0

Background Information

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL17A, IL-17A, interleukin 17A