B4GALT1 Polyclonal antibody

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  WB analysis using 55448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55448-1-AP (B4GALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

B4GALT1 Polyclonal Antibody for WB, ELISA

Cat No. 55448-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

GTB, GT1, GGTB2, beta4Gal T1, Beta 1,4 GalTase 1

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, mouse small intestine tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55448-1-AP targets B4GALT1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: NM_001497
• Gene Symbol: B4GALT1
• Gene ID (NCBI): 2683
• RRID: AB_3670253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15291
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B4GALT1 is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. B4GALT1 is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. B4GALT1 has two isoforms with molecular weights of 44 kda and 43 kda that are localized to the cell surface and Golgi apparatus, respectively.