Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, human brain tissue, mouse pancreas tissue, HeLa cells, zebrafish tissue, PC-12 cells, rat brain tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|mouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:100-1:400
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12807-1-AP targets BACE1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.
|human, mouse, zebrafish, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|BACE1 fusion protein Ag3559
|beta-site APP-cleaving enzyme 1
|Calculated molecular weight
|501 aa, 56 kDa
|Observed molecular weight
|70-75 kDa, 42-56 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).
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Xiaoyu (Verified Customer) (02-07-2024)