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  • KD/KO Validated

BACE1 Polyclonal antibody

BACE1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12807-1-AP

Synonyms

Asp 2, ASP2, Aspartyl protease 2, BACE, BACE1, Beta secretase 1, HSPC104, KIAA1149, Memapsin 2



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse pancreas tissue, HeLa cells, zebrafish tissue, PC-12 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12807-1-AP targets BACE1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.

Tested Reactivity human, mouse, zebrafish, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BACE1 fusion protein Ag3559
Full Name beta-site APP-cleaving enzyme 1
Calculated molecular weight 501 aa, 56 kDa
Observed molecular weight 70-75 kDa, 42-56 kDa
GenBank accession numberBC036084
Gene symbol BACE1
Gene ID (NCBI) 23621
RRIDAB_10638760
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).

Protocols

Product Specific Protocols
WB protocol for BACE1 antibody 12807-1-APDownload protocol
IHC protocol for BACE1 antibody 12807-1-APDownload protocol
IP protocol for BACE1 antibody 12807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Small

Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.

Authors - Qin Ouyang
mouseWB

Redox Biol

Hyperoside alleviates toxicity of β-amyloid via endoplasmic reticulum-mitochondrial calcium signal transduction cascade in APP/PS1 double transgenic Alzheimer's disease mice

Authors - Lin Lin Song
mouseWB

Redox Biol

A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease

Authors - Yi Sun
mouseWB

Proc Natl Acad Sci U S A

Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.

Authors - Gunjan Joshi
human,mouseWB

Cell Rep

CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.

Authors - Yang-Ping Shentu
mouseWB

Stem Cell Reports

BACE1 Deficiency Causes Abnormal Neuronal Clustering in the Dentate Gyrus.

Authors - Hailong Hou
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: brain
BACE1 Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:12807-1-AP)