BAD Monoclonal antibody

BAD Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4B12G2

Cat no : 67830-1-Ig

Synonyms

BAD, BBC2, BBC6

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Validation Data Gallery

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  WB analysis using 67830-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 67830-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, RAW 264.7 cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, THP-1 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

67830-1-Ig targets BAD in WB,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: BCL2-associated agonist of cell death
• Calculated molecular weight: 18 kDa
• Observed molecular weight: 18-25 kDa
• GenBank accession number: BC001901
• Gene symbol: BAD
• Gene ID (NCBI): 572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Publications

Species	Application	Title
human	WB	FASEB J; Glucose-dependent insulinotropic polypeptide modifies adipose plasticity and promotes beige adipogenesis of human omental adipose-derived stem cells.; Authors - Xueying Chen