BAD Monoclonal antibody

BAD Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B12G2

Cat no : 67830-1-Ig

Synonyms

BAD, BBC2, BBC6



Tested Applications

Positive WB detected inLNCaP cells, RAW 264.7 cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, THP-1 cells, HSC-T6 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67830-1-Ig targets BAD in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name BCL2-associated agonist of cell death
Calculated molecular weight 18 kDa
Observed molecular weight18-25 kDa
GenBank accession numberBC001901
Gene symbol BAD
Gene ID (NCBI) 572
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Protocols

Product Specific Protocols
WB protocol for BAD antibody 67830-1-IgDownload protocol
IF protocol for BAD antibody 67830-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Glucose-dependent insulinotropic polypeptide modifies adipose plasticity and promotes beige adipogenesis of human omental adipose-derived stem cells.

Authors - Xueying Chen
ratWB

Oxid Med Cell Longev

Curcumin Inhibits Hyperandrogen-Induced IRE1α-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats

Authors - Yaling Zhang