DU 145 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP(BAG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10599-1-AP(BAG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP(BAG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP(BAG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP(BAG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse uterus tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP(BAG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP( BAG3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10599-1-AP( BAG3 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10599-1-AP( BAG3 Antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-BAG3 (IP:10599-1-AP, 4ug; Detection:10599-1-AP 1:1000) with K-562 cells lysate 11000ug.

Immunofluorescent analysis of Hela cells, using BAG3 antibody 10599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).