• Featured Product
  • KD/KO Validated

BAG3 Polyclonal antibody

BAG3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 10599-1-AP


BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, mouse heart, rat heart
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, SH-SY5Y cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10599-1-AP targets BAG3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG3 fusion protein Ag0956
Full Name BCL2-associated athanogene 3
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 74-80 kDa
GenBank Accession NumberBC006418
Gene Symbol BAG3
Gene ID (NCBI) 9531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.


Product Specific Protocols
WB protocol for BAG3 antibody 10599-1-APDownload protocol
IHC protocol for BAG3 antibody 10599-1-APDownload protocol
IF protocol for BAG3 antibody 10599-1-APDownload protocol
IP protocol for BAG3 antibody 10599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Genet

Mutations affecting the cytoplasmic functions of the co-chaperone DNAJB6 cause limb-girdle muscular dystrophy.

Authors - Sarparanta Jaakko J

Nat Neurosci

A tau homeostasis signature is linked with the cellular and regional vulnerability of excitatory neurons to tau pathology.

Authors - Hongjun Fu
  • KD Validated

Acta Neuropathol

Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions.

Authors - Mridul Johari

J Intern Med

Transglutaminase type 2 plays a key role in the pathogenesis of Mycobacterium tuberculosis infection.

Authors - I Palucci

Nat Commun

Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex.

Authors - Joori Park
humanWB, IF

Nat Commun

Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.

Authors - Thomas G Martin


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Andrea (Verified Customer) (01-03-2023)

Strong, clear band on the membrane using a 1:500 ratio.

  • Applications: Western Blot

WEI (Verified Customer) (03-03-2022)

Very strong antibody, confirmed by KO tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse Heart

Jane (Verified Customer) (02-03-2022)

One specific band at 80kDa in mouse heart tissue lysate

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Heart/cardiomyocytes

Eric (Verified Customer) (01-25-2021)

The red stain is 1:100 BAG3 under laser confocal microscopy in human microglia cells.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Microglia
BAG3 Antibody Western Blot,Immunofluorescence validation (1:100 dilution) in Human Microglia (Cat no:10599-1-AP)

Tongbin (Verified Customer) (08-25-2020)

This bag3 antibody is very specific without any non-specific bands. It is the best Bag3 antibody out there for western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
BAG3 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:10599-1-AP)

Afaque (Verified Customer) (10-17-2019)

I have used this antibody to study autophagy. It has worked very well for my Western blots. I also tried for IF and got very good results.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Cardiomyocytes
BAG3 Antibody Western Blot,Immunofluorescence, validation (1:3000 dilution) in Cardiomyocytes (Cat no:10599-1-AP)

SHUBHAM (Verified Customer) (02-25-2019)

excellent Antibody

  • Applications: Western Blot, Immunohistochemistry, Cell culture,
  • Cell Tissue Type: AC16
BAG3 Antibody Western Blot,Immunohistochemistry,Cell culture, validation ( dilution) in AC16 (Cat no:10599-1-AP)

Praveen (Verified Customer) (12-03-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Brain,(Rat), Heart, Skeletal muscles, lung, Kidney etc (Mice), Human Cardio myocytes