BAG3 Polyclonal antibody

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  WB analysis using 10599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10599-1-AP

  IP result of anti-BAG3 (IP:10599-1-AP, 4ug; Detection:10599-1-AP 1:1000) with K-562 cells lysate 11000ug.

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  IHC staining of human gliomas using 10599-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10599-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 10599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 10599-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 10599-1-AP

  Immunofluorescent analysis of Hela cells, using BAG3 antibody 10599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

BAG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10599-1-AP

Publications(125)

Reviews (8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

MFM6, Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, mouse heart, rat heart
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human lung cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:30000-1:60000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 27 publications below
• WB: See 109 publications below
• IHC: See 6 publications below
• IF: See 47 publications below
• IP: See 6 publications below
• FC: See 1 publications below
• CoIP: See 3 publications below

Product Information

10599-1-AP targets BAG3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAG3 fusion protein Ag0956
• Full Name: BCL2-associated athanogene 3
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 74-80 kDa
• GenBank Accession Number: BC006418
• Gene Symbol: BAG3
• Gene ID (NCBI): 9531
• RRID: AB_2062602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95817
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.

Publications

Species	Application	Title
monkey	WB,IHC	Nat Genet; Mutations affecting the cytoplasmic functions of the co-chaperone DNAJB6 cause limb-girdle muscular dystrophy.; Authors - Sarparanta Jaakko J
human	WB	Nat Neurosci; A tau homeostasis signature is linked with the cellular and regional vulnerability of excitatory neurons to tau pathology.; Authors - Hongjun Fu ; KD Validated
human	IF	Acta Neuropathol; Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions.; Authors - Mridul Johari
mouse	WB	J Intern Med; Transglutaminase type 2 plays a key role in the pathogenesis of Mycobacterium tuberculosis infection.; Authors - I Palucci
human	WB	Nat Commun; Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex.; Authors - Joori Park
human	WB, IF	Nat Commun; Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.; Authors - Thomas G Martin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (01-03-2023)

Strong, clear band on the membrane using a 1:500 ratio.

• Applications: Western Blot

FH

WEI (Verified Customer) (03-03-2022)

Very strong antibody, confirmed by KO tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse Heart

FH

Jane (Verified Customer) (02-03-2022)

One specific band at 80kDa in mouse heart tissue lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Heart/cardiomyocytes

FH

Eric (Verified Customer) (01-25-2021)

The red stain is 1:100 BAG3 under laser confocal microscopy in human microglia cells.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Microglia

BAG3 Antibody Western Blot,Immunofluorescence validation (1:100 dilution) in Human Microglia (Cat no:10599-1-AP)

FH

Tongbin (Verified Customer) (08-25-2020)

This bag3 antibody is very specific without any non-specific bands. It is the best Bag3 antibody out there for western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

BAG3 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:10599-1-AP)

FH

Afaque (Verified Customer) (10-17-2019)

I have used this antibody to study autophagy. It has worked very well for my Western blots. I also tried for IF and got very good results.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Cardiomyocytes

BAG3 Antibody Western Blot,Immunofluorescence, validation (1:3000 dilution) in Cardiomyocytes (Cat no:10599-1-AP)

FH

SHUBHAM (Verified Customer) (02-25-2019)

excellent Antibody

• Applications: Western Blot, Immunohistochemistry, Cell culture,
• Cell Tissue Type: AC16

BAG3 Antibody Western Blot,Immunohistochemistry,Cell culture, validation ( dilution) in AC16 (Cat no:10599-1-AP)

FH

Praveen (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Brain,(Rat), Heart, Skeletal muscles, lung, Kidney etc (Mice), Human Cardio myocytes