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- KD/KO Validated
BAG3 Polyclonal antibody
BAG3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10599-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, mouse heart, rat heart |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SH-SY5Y cells, HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 20 publications below |
| WB | See 72 publications below |
| IHC | See 4 publications below |
| IF | See 35 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
10599-1-AP targets BAG3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BAG3 fusion protein Ag0956 |
| Full Name | BCL2-associated athanogene 3 |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 74-80 kDa |
| GenBank accession number | BC006418 |
| Gene symbol | BAG3 |
| Gene ID (NCBI) | 9531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAG3 antibody 10599-1-AP | Download protocol |
| IHC protocol for BAG3 antibody 10599-1-AP | Download protocol |
| IF protocol for BAG3 antibody 10599-1-AP | Download protocol |
| IP protocol for BAG3 antibody 10599-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy BAG3 and SYNPO (synaptopodin) facilitate phospho-MAPT/Tau degradation via autophagy in neuronal processes.
| ||
Redox Biol Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress.
| ||
Sci Rep Role of Bcl2-associated Athanogene 3 in Turnover of Gap Junction Protein, Connexin 43, in Neonatal Cardiomyocytes.
| ||
Cell Death Dis Perturbation of synapsins homeostasis through HIV-1 Tat-mediated suppression of BAG3 in primary neuronal cells.
| ||
Neurobiol Aging Nrf2 mediates the expression of BAG3 and autophagy cargo adaptor proteins and tau clearance in an age-dependent manner. | ||
J Biol Chem Silencing of the Hsp70-specific nucleotide-exchange factor BAG3 corrects the F508del-CFTR variant by restoring autophagy.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH WEI (Verified Customer) (03-03-2022) | Very strong antibody, confirmed by KO tissue
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FH Jane (Verified Customer) (02-03-2022) | One specific band at 80kDa in mouse heart tissue lysate
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FH Eric (Verified Customer) (01-25-2021) | The red stain is 1:100 BAG3 under laser confocal microscopy in human microglia cells.
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FH Tongbin (Verified Customer) (08-25-2020) | This bag3 antibody is very specific without any non-specific bands. It is the best Bag3 antibody out there for western blots.
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FH Afaque (Verified Customer) (10-17-2019) | I have used this antibody to study autophagy. It has worked very well for my Western blots. I also tried for IF and got very good results.
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FH SHUBHAM (Verified Customer) (02-25-2019) | excellent Antibody
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FH Praveen (Verified Customer) (12-03-2018) |
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