BAG3 Monoclonal antibody

BAG3 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rabbit, pig, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4G6C2

Cat no : 68076-1-Ig

Synonyms

BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

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Validation Data Gallery

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  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 68076-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using BAG3 antibody (68076-1-Ig, Clone: 4G6C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, LNCaP cells, rabbit heart tissue, HeLa cells, pig heart, rat heart
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

68076-1-Ig targets BAG3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rabbit, pig, rat samples.

• Tested Reactivity: Human, mouse, rabbit, pig, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BAG3 fusion protein Ag17608
• Full Name: BCL2-associated athanogene 3
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC006418
• Gene Symbol: BAG3
• Gene ID (NCBI): 9531
• RRID: AB_2918814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.

Publications

Species	Application	Title
human,rat	IHC	J Physiol Biochem; BAG3 protects chondrocytes against lumbar facet joint osteoarthritis by regulating autophagy and apoptosis.; Authors - Xin Lu
rat	WB,IF	J Physiol Sci; High-intensity interval training alleviates exhaustive exercise-induced HSP70-assisted selective autophagy in skeletal muscle; Authors - Jiao Lu