BAG3 Monoclonal antibody

BAG3 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rabbit, pig, rat and More (1)







Cat no : 68076-1-Ig


BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

Tested Applications

Positive WB detected inNIH/3T3 cells, LNCaP cells, rabbit heart tissue, HeLa cells, pig heart, rat heart
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68076-1-Ig targets BAG3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rabbit, pig, rat samples.

Tested Reactivity Human, mouse, rabbit, pig, rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BAG3 fusion protein Ag17608
Full Name BCL2-associated athanogene 3
Calculated Molecular Weight 61 kDa
Observed Molecular Weight74 kDa
GenBank Accession NumberBC006418
Gene Symbol BAG3
Gene ID (NCBI) 9531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.


Product Specific Protocols
WB protocol for BAG3 antibody 68076-1-IgDownload protocol
IF protocol for BAG3 antibody 68076-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Physiol Biochem

BAG3 protects chondrocytes against lumbar facet joint osteoarthritis by regulating autophagy and apoptosis.

Authors - Xin Lu

J Physiol Sci

High-intensity interval training alleviates exhaustive exercise-induced HSP70-assisted selective autophagy in skeletal muscle

Authors - Jiao Lu