BAHCC1 Polyclonal antibody

BAHCC1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30046-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, IF/ICC, ELISA

BAHCC1, BAHD2, FLJ23058, KIAA1447

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inmouse brain tissue, rat brain tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30046-1-AP targets BAHCC1 in IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAHCC1 fusion protein Ag32599 Predict reactive species
Full Name BAH domain and coiled-coil containing 1
Calculated Molecular Weight280 kDa
Observed Molecular Weight 280 kDa
GenBank Accession NumberNM_001291324.1
Gene Symbol BAHCC1
Gene ID (NCBI) 57597
RRIDAB_3086218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P281
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAHCC1 (BAH and coiled-coil domain-containing protein 1) is also named as BAHD2, KIAA1447 and BAH domain-containing protein 2. BAHCC1 is a transcriptional regulator controlling expression of E2F/KLF-dependent cell-cycle and DNA-repair genes. BAHCC1 associates with BRG1-containing remodeling complexes at the promoters of these genes. BAHCC1 silencing leads to decreased cell proliferation and delayed DNA repair. Consequently, BAHCC1 deficiency cooperates with PARP inhibition to induce melanoma cell death (PMID: 37924516). depletion of BAHCC1, or disruption of the BAHCC1BAH-H3K27me3 interaction, causes derepression of H3K27me3-targeted genes that are involved in tumor suppression and cell differentiation, leading to suppression of oncogenesis (PMID: 33139953).

Protocols

Product Specific Protocols
IHC protocol for BAHCC1 antibody 30046-1-APDownload protocol
IF protocol for BAHCC1 antibody 30046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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