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BAZ2A antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, WB

Activ Motif logo

Cat No : 61707,61708 61707

Synonyms

BAZ2A, Bromodomain Adjacent To Zinc Finger Domain 2A, TTF-I-Interacting Protein 5, TTF-I Interacting Peptide 5, WALp3, Transcription Termination Factor I-Interacting Protein 5, Bromodomain Adjacent To Zinc Finger Domain Protein 2A, TIP5, KIAA0314, hWALp3, Tip5, NoRC complex, Transcription silencing, polyclonal, pab, antibody, antibodies, western blotting, wb, sample

Validation Data Gallery

  • BAZ2A antibody (pAb) tested by Western blot. BAZ2A antibody detection by Western blot. The analysis was performed using 40 ug of HeLa nuclear extract and BAZ2A at a 1:500 dilution.

    BAZ2A antibody (pAb) tested by Western blot. BAZ2A antibody detection by Western blot. The analysis was performed using 40 ug of HeLa nuclear extract and BAZ2A at a 1:500 dilution.

Product Information

Tested Applications IF, WB

Applications Validated by Active Motif: WB*: 1:500 - 1:1,000 dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human BAZ2A.
Full Name BAZ2A antibody (pAb)
Synonyms BAZ2A, Bromodomain Adjacent To Zinc Finger Domain 2A, TTF-I-Interacting Protein 5, TTF-I Interacting Peptide 5, WALp3, Transcription Termination Factor I-Interacting Protein 5, Bromodomain Adjacent To Zinc Finger Domain Protein 2A, TIP5, KIAA0314, hWALp3, Tip5, NoRC complex, Transcription silencing, polyclonal, pab, antibody, antibodies, western blotting, wb, sample
Molecular weight 270 kDa
GenBank accession numberNP_038477
RRIDAB_2793744
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

BAZ2A (Bromodomain Adjacent To Zinc Finger Domain, 2A) is an essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).