BBS2 Polyclonal antibody

BBS2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 11188-2-AP

Synonyms

Bardet Biedl syndrome 2, BBS, BBS2

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Validation Data Gallery

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  WB analysis using 11188-2-AP

  Mouse testis lysates were subjected to SDS PAGE followed by western blot with 11188-2-AP (BBS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11188-2-AP

  IP Result of anti-BBS2 (IP:11188-2-AP, 4ug; Detection:11188-2-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of hTERT-RPE1 cells using 11188-2-AP

  IF result (cilia stain) of anti-BBS2 (11188-2-AP, 1:50) with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IF detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Product Information

11188-2-AP targets BBS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS2 fusion protein Ag1662
• Full Name: Bardet-Biedl syndrome 2
• Calculated molecular weight: 80 kDa
• Observed molecular weight: 80 kDa
• GenBank accession number: BC014140
• Gene symbol: BBS2
• Gene ID (NCBI): 583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.

Publications

Species	Application	Title
human	IF	Cell; The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.; Authors - Jin Hua H
mouse	IF	Cell Stem Cell; Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.; Authors - Kusek Gretchen G
mouse	IF	J Cell Biol; Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.; Authors - Paurav B Desai
mouse	IHC	Brain Struct Funct; A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice.; Authors - Peter Acs
mouse	IF	Hum Mol Genet; Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.; Authors - Jiang Si-Tse ST
mouse	IF	FASEB J; Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.; Authors - Wei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deng (Verified Customer) (07-30-2019)

This antibody stained primary cilia in cultured IMCD3 cells.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1;250
• Cell Tissue Type: IMCD3 cells

BBS2 Antibody Immunofluorescence, validation (1;250 dilution) in IMCD3 cells (Cat no:11188-2-AP)