BBS2 Polyclonal antibody

BBS2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11188-2-AP

Synonyms

Bardet Biedl syndrome 2, BBS, BBS2



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11188-2-AP targets BBS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS2 fusion protein Ag1662
Full Name Bardet-Biedl syndrome 2
Calculated molecular weight 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC014140
Gene symbol BBS2
Gene ID (NCBI) 583
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.

Protocols

Product Specific Protocols
WB protocol for BBS2 antibody 11188-2-APDownload protocol
IP protocol for BBS2 antibody 11188-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.

Authors - Jin Hua H
mouseIF

Cell Stem Cell

Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.

Authors - Kusek Gretchen G
mouseIF

J Cell Biol

Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.

Authors - Paurav B Desai
mouseIHC

Brain Struct Funct

A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice.

Authors - Peter Acs
mouseIF

Hum Mol Genet

Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.

Authors - Jiang Si-Tse ST
mouseIF

FASEB J

Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Authors - Wei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Deng (Verified Customer) (07-30-2019)

This antibody stained primary cilia in cultured IMCD3 cells.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1;250
  • Cell Tissue Type: IMCD3 cells
BBS2 Antibody Immunofluorescence, validation (1;250 dilution) in IMCD3 cells (Cat no:11188-2-AP)