BBS2 Polyclonal antibody

BBS2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11188-2-AP

Synonyms

Bardet Biedl syndrome 2, BBS, BBS2



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11188-2-AP targets BBS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS2 fusion protein Ag1662
Full Name Bardet-Biedl syndrome 2
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC014140
Gene Symbol BBS2
Gene ID (NCBI) 583
RRIDAB_2064971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.

Protocols

Product Specific Protocols
WB protocol for BBS2 antibody 11188-2-APDownload protocol
IP protocol for BBS2 antibody 11188-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.

Authors - Jin Hua H
mouseIF

Cell Stem Cell

Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.

Authors - Kusek Gretchen G
mouseIF

J Cell Biol

Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.

Authors - Paurav B Desai
mouseIHC

Brain Struct Funct

A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice.

Authors - Peter Acs
mouseIF

Hum Mol Genet

Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.

Authors - Jiang Si-Tse ST
mouseIF

FASEB J

Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Authors - Wei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Deng (Verified Customer) (07-30-2019)

This antibody stained primary cilia in cultured IMCD3 cells.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1;250
  • Cell Tissue Type: IMCD3 cells
BBS2 Antibody Immunofluorescence, validation (1;250 dilution) in IMCD3 cells (Cat no:11188-2-AP)