BBS6 Polyclonal antibody

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  WB analysis of mouse testis using 13078-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13078-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 cells using 13078-1-AP

  IF result (cytoplasm and the base of cilia stain) of anti-BBS6 (13078-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

BBS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13078-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MKKS, MKS, McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin, KMS, HMCS

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13078-1-AP targets BBS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS6 fusion protein Ag3785
• Full Name: McKusick-Kaufman syndrome
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC028973
• Gene Symbol: MKKS
• Gene ID (NCBI): 8195
• RRID: AB_10603370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NPJ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKKS also known as BBS6 is a probable chaperone given to the amino acid similarity to the chaperonin family of proteins and may play a role in protein processing in limb, cardiac and reproductive system development. The mutations in BBS6 have been linked to Bardet-Biedl syndrome (BBS) which is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. It may also get involved in cellular organization processes, in particular relating to ciliary/flagellar and centrosomal activities.

Publications

Species	Application	Title
mouse	WB	Mol Neurobiol; Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure; Authors - Xiuwei Wang