BBS7 Polyclonal antibody
BBS7 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
WB, IP, IHC,ELISA
Cat no : 18961-1-AP
Validation Data Gallery
|Positive WB detected in||human brain tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human testis tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|WB||See 7 publications below|
|IHC||See 1 publications below|
|IP||See 2 publications below|
18961-1-AP targets BBS7 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BBS7 fusion protein Ag4601|
|Full Name||Bardet-Biedl syndrome 7|
|Calculated molecular weight||715 aa, 80 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC032691|
|Gene ID (NCBI)||55212|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.
|Product Specific Protocols|
|WB protocol for BBS7 antibody 18961-1-AP||Download protocol|
|IHC protocol for BBS7 antibody 18961-1-AP||Download protocol|
|IP protocol for BBS7 antibody 18961-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.
A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.
The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome.
BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.
Hum Mol Genet
Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome.
J Biol Chem
The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.