BBS7 Polyclonal antibody

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  WB analysis of human brain using 18961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 18961-1-AP

  IP result of anti-BBS7 (IP:18961-1-AP, 4ug; Detection:18961-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens).

BBS7 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 18961-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Bardet Biedl syndrome 7, BBS2 like protein 1, BBS2L1, BBS7

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

18961-1-AP targets BBS7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS7 fusion protein Ag4601
• Full Name: Bardet-Biedl syndrome 7
• Calculated Molecular Weight: 715 aa, 80 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC032691
• Gene Symbol: BBS7
• Gene ID (NCBI): 55212
• RRID: AB_2064998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWZ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	PLoS Genet; A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.; Authors - Seo Seongjin S
human	WB	PLoS Genet; The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome.; Authors - Xitiz Chamling
mouse	WB	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
mouse	IHC, IP, WB	Hum Mol Genet; Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome.; Authors - Ying Hsu
mouse	IF	FASEB J; Rhodopsin mislocalization drives ciliary dysregulation in a novel autosomal dominant retinitis pigmentosa knock-in mouse model; Authors - Shimpei Takita