BBS7 Polyclonal antibody
BBS7 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC,ELISA
Conjugate
Unconjugated
Cat no : 18961-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 7 publications below |
IHC | See 1 publications below |
IP | See 2 publications below |
Product Information
18961-1-AP targets BBS7 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | BBS7 fusion protein Ag4601 |
Full Name | Bardet-Biedl syndrome 7 |
Calculated molecular weight | 715 aa, 80 kDa |
Observed molecular weight | 75 kDa |
GenBank accession number | BC032691 |
Gene symbol | BBS7 |
Gene ID (NCBI) | 55212 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for BBS7 antibody 18961-1-AP | Download protocol |
IHC protocol for BBS7 antibody 18961-1-AP | Download protocol |
IP protocol for BBS7 antibody 18961-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. | ||
PLoS Genet A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened. | ||
PLoS Genet The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome. | ||
PLoS Genet BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment. | ||
Hum Mol Genet Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome. | ||
J Biol Chem The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. |