BBS7 Polyclonal antibody

BBS7 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18961-1-AP

Synonyms

Bardet Biedl syndrome 7, BBS2 like protein 1, BBS2L1, BBS7



Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18961-1-AP targets BBS7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS7 fusion protein Ag4601
Full Name Bardet-Biedl syndrome 7
Calculated molecular weight 715 aa, 80 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC032691
Gene symbol BBS7
Gene ID (NCBI) 55212
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.

Protocols

Product Specific Protocols
WB protocol for BBS7 antibody 18961-1-APDownload protocol
IHC protocol for BBS7 antibody 18961-1-APDownload protocol
IP protocol for BBS7 antibody 18961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Invest Ophthalmol Vis Sci

Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.

Authors - Satyabrata Sinha
mouseWB,IP

J Biol Chem

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

Authors - Poppy Datta
mouseWB

PLoS Genet

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

Authors - Ying Hsu
mouseWB

PLoS Genet

A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.

Authors - Seo Seongjin S
humanWB

PLoS Genet

The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome.

Authors - Xitiz Chamling
mouseWB

Proc Natl Acad Sci U S A

Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.

Authors - Poppy Datta