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  • KD/KO Validated

BCAT1 Polyclonal antibody

BCAT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13640-1-AP

Synonyms

BCAT1, BCAT1/ECA39, ECA39, MECA39, PNAS 121, PP18, Protein ECA39



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Neuro-2a cells
Positive IHC detected inmouse brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13640-1-AP targets BCAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAT1 fusion protein Ag4574
Full Name branched chain aminotransferase 1, cytosolic
Calculated Molecular Weight 320 aa, 36 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2063569
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.

Protocols

Product Specific Protocols
WB protocol for BCAT1 antibody 13640-1-APDownload protocol
IHC protocol for BCAT1 antibody 13640-1-APDownload protocol
IF protocol for BCAT1 antibody 13640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Metab

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression

Authors - Lin Qian
  • KO Validated
mouseIHC

Nat Cell Biol

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Authors - Jin-Tao Li
humanWB, IHC

Basic Res Cardiol

Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.

Authors - Qiong Lai
humanWB

Cancer Res

Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.

Authors - Bo Zhang
humanWB

Cell Mol Life Sci

Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.

Authors - Bo Zhang
  • KO Validated
humanWB,IHC

Oncotarget

BCAT1 expression associates with ovarian cancer progression: possible implications in altered disease metabolism.

Authors - Zhi-Qiang Wang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kis (Verified Customer) (12-08-2022)

This antibody was used for (1:1000) WB and (1:500) for IHC. We got satisfactory results with this ab.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells
BCAT1 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Human Liver cells (Cat no:13640-1-AP)
FH

Iru (Verified Customer) (06-18-2020)

It works fine with FTE cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells