Tested Applications
| Positive WB detected in | Jurkat cells, Daudi cells, HeLa cells, mouse brain tissue, rat brain tissue, Neuro-2a cells |
| Positive IHC detected in | mouse brain tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13640-1-AP targets BCAT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4574 Product name: Recombinant human BCAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC033864 Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF Predict reactive species |
| Full Name | branched chain aminotransferase 1, cytosolic |
| Calculated Molecular Weight | 320 aa, 36 kDa |
| Observed Molecular Weight | 43-45 kDa |
| GenBank Accession Number | BC033864 |
| Gene Symbol | BCAT1 |
| Gene ID (NCBI) | 586 |
| RRID | AB_2063569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54687 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCAT1, also named branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine, and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). The 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BCAT1 antibody 13640-1-AP | Download protocol |
| IHC protocol for BCAT1 antibody 13640-1-AP | Download protocol |
| WB protocol for BCAT1 antibody 13640-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-BCAT1 antibody (Cat# 13640-1-AP) has 59 citations published in journals including Nature, Nature Metabolism, Nature Methods, Nature Communications, Cell Metabolism, Nature Cell Biology, Cancer Research, Advanced Science, Cell Death & Disease, Phytomedicine, and others. Research fields span BCAA metabolism, oncology (glioblastoma, pancreatic, gastric, lung, ovarian cancers), ferroptosis, cardiovascular disease, neurodegeneration, fibrosis, diabetes, and inflammatory disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Branched-chain amino acid transaminase 1 confers EGFR-TKI resistance through epigenetic glycolytic activation | ||
Cell Metab Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis | ||
Nat Metab Branched-chain α-ketoacids aerobically activate HIF1α signalling in vascular cells | ||
Nat Metab Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression
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Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1000 dilution. One user (5 stars) also tested it for IHC at 1:500 on human liver cells and reported satisfactory results overall. The other (3 stars) confirmed it works fine with fallopian tube epithelial cells. In summary, the antibody performs reliably for WB across different cell types, with one positive IHC report as well.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kis (Verified Customer) (12-08-2022) | This antibody was used for (1:1000) WB and (1:500) for IHC. We got satisfactory results with this ab.
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Iru (Verified Customer) (06-18-2020) | It works fine with FTE cells.
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