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BDNF Polyclonal antibody
BDNF Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
human, rat and More (2)
WB, IHC, IF, ELISA
Cat no : 25699-1-AP
Validation Data Gallery
|Positive WB detected in||fetal human brain tissue, U-251 cells, rat brain tissue|
|Western Blot (WB)||WB : 1:200-1:1000|
|Sample-dependent, check data in validation data gallery|
|WB||See 21 publications below|
|IHC||See 10 publications below|
|IF||See 3 publications below|
The immunogen of 25699-1-AP is BDNF Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, mouse, rat, macaque|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BDNF fusion protein Ag22489|
|Full Name||brain-derived neurotrophic factor|
|Calculated molecular weight||247 aa, 28 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC029795|
|Gene ID (NCBI)||627|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
1) What is BDNF?
BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.
2) FAQs for BDNF
A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).
Early BDNF Treatment Ameliorates Cell Loss in the Entorhinal Cortex of APP Transgenic Mice.
CPEB3-dowregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice.
Cell Mol Life Sci
Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.
Neuroprotective and neuro-rehabilitative effects of acute purinergic receptor P2X4 (P2X4R) blockade after ischemic stroke.
Danshen-Chuanxiong-Honghua Ameliorates Cerebral Impairment and Improves Spatial Cognitive Deficits after Transient Focal Ischemia and Identification of Active Compounds.
J Cell Physiol
miR-124 and miR-9 Mediated Downregulation of HDAC5 Promotes Neurite Development Through Activating MEF2C- GPM6A Pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Armelle (Verified Customer) (09-03-2019)
This antibody worked very well. We were able to quantify expression levels in our area of interest.