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  • KD/KO Validated

BDNF Polyclonal antibody

BDNF Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25699-1-AP

Synonyms

Abrineurin, BDNF



Tested Applications

Positive WB detected infetal human brain tissue, U-251 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25699-1-AP is BDNF Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, macaque
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BDNF fusion protein Ag22489
Full Name brain-derived neurotrophic factor
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC029795
Gene symbol BDNF
Gene ID (NCBI) 627
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publications

SpeciesApplicationTitle
mouseIHC

J Neurosci

Early BDNF Treatment Ameliorates Cell Loss in the Entorhinal Cortex of APP Transgenic Mice.

Authors - Nagahara Alan H AH
mouseWB

Neuropsychopharmacology

CPEB3-dowregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice.

Authors - Wen-Hsin Lu
mouseWB

Cell Mol Life Sci

Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.

Authors - Binglin Zhu
mouseWB

Exp Neurol

Neuroprotective and neuro-rehabilitative effects of acute purinergic receptor P2X4 (P2X4R) blockade after ischemic stroke.

Authors - Pranay Srivastava
mouseWB

Front Pharmacol

Danshen-Chuanxiong-Honghua Ameliorates Cerebral Impairment and Improves Spatial Cognitive Deficits after Transient Focal Ischemia and Identification of Active Compounds.

Authors - Xianhua Zhang
humanWB

J Cell Physiol

miR-124 and miR-9 Mediated Downregulation of HDAC5 Promotes Neurite Development Through Activating MEF2C- GPM6A Pathway.

Authors - Xi Gu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Armelle (Verified Customer) (09-03-2019)

This antibody worked very well. We were able to quantify expression levels in our area of interest.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: bain slice fom the ventrolateral hypothalamus
BDNF Antibody Immunofluorescence, validation (1/500 dilution) in bain slice fom the ventrolateral hypothalamus (Cat no:25699-1-AP)