Biglycan Polyclonal antibody

• Featured Product
• KD/KO Validated

Biglycan Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

25

Reviews (2)

Cat no : 16409-1-AP

Synonyms

BGN, PRG1, PG-S1, PGI, PG S1

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Validation Data Gallery

• 

  WB analysis using 16409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16409-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cartilage using 16409-1-AP

  pig cartilage tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16409-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16409-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human normal colon using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16409-1-AP (Biglycan antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16409-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Biglycan antibody (16409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue, pig cartilage tissue
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human kidney tissue, human liver tissue, human liver cancer tissue, human lung tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:200-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 7 publications below

Product Information

16409-1-AP targets Biglycan in WB, IHC, IF/ICC, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Biglycan fusion protein Ag0683
• Full Name: biglycan
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 40-48 kDa
• GenBank Accession Number: BC004244
• Gene Symbol: Biglycan
• Gene ID (NCBI): 633
• RRID: AB_10804656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Publications

Species	Application	Title
human	WB,IHC	Oncoimmunology; Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer.; Authors - Shaoquan Zheng
human	WB,IF	Cancers (Basel); Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.; Authors - Kanakaraju Manupati ; KD Validated
mouse	IHC	J Biol Eng; Proteomic analysis of decellularized mice liver and kidney extracellular matrices; Authors - Anna-Maria Diedrich
human	WB,IHC	Oncoimmunology; Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.; Authors - Karthikeyan Subbarayan
rat	IF	Cell Prolif; Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.; Authors - Yunjiao Wang
human	IHC	Cancer Med; Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma; Authors - Wanyi Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lin (Verified Customer) (11-18-2024)

Cells were fixed in 4% PFA, permeabilized with .1% Triton, and blocked for 1 hour. 1o was incubated overnight in 1% BSA followed by 2o incubation for 1hr and imaging. More concentrated dilutions than 1:500 (eg 1:200) produced nonspecific aggregates outside of cells that were not seen in 2o only control.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MC3T3 cells (mouse pre-osteoblasts), not treated with Ascorbic acid

FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chronogenic Kit Antigen Retrival ER1 (ph6) for 30 min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Placenta healthy

Biglycan Antibody Immunohistochemistry validation (1:500 dilution) in Placenta healthy (Cat no:16409-1-AP)

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