Biglycan Polyclonal antibody

Biglycan Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16409-1-AP

Synonyms

A6NLG9, BGN, biglycan, Bone/cartilage proteoglycan I, DSPG1, PG S1, PGI, SLRR1A



Tested Applications

Positive WB detected inCOLO 320 cells, mouse skeletal muscle tissue, HeLa cells, HepG2 cells, pig cartilage tissue
Positive IHC detected inhuman lung tissue, human liver cancer tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16409-1-AP is Biglycan Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Biglycan fusion protein Ag0683
Full Name biglycan
Calculated molecular weight 42 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC004244
Gene symbol BGN
Gene ID (NCBI) 633
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Protocols

Product Specific Protocols
WB protocol for Biglycan antibody 16409-1-APDownload protocol
IHC protocol for Biglycan antibody 16409-1-APDownload protocol
IF protocol for Biglycan antibody 16409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway.

Authors - Qi Cui
humanWB

Proteomics Clin Appl

Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction.

Authors - Yueyan Li
humanIHC

Thorac Cancer

Biglycan, tumor endothelial cell secreting proteoglycan, as possible biomarker for lung cancer.

Authors - Hirofumi Morimoto
humanWB

Glycoconj J

Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency.

Authors - Mengying Wang
ratIF

Cell Prolif

Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.

Authors - Yunjiao Wang
humanWB,IHC

Oncoimmunology

Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.

Authors - Karthikeyan Subbarayan