• Featured Product
  • KD/KO Validated

Biglycan Polyclonal antibody

Biglycan Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 16409-1-AP

Synonyms

A6NLG9, BGN, biglycan, Bone/cartilage proteoglycan I, DSPG1, PG S1, PGI, SLRR1A



Tested Applications

Positive WB detected inCOLO 320 cells, mouse skeletal muscle tissue, HeLa cells, HepG2 cells, pig cartilage tissue
Positive IHC detected inhuman lung tissue, human liver cancer tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16409-1-AP is Biglycan Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Biglycan fusion protein Ag0683
Full Name biglycan
Calculated molecular weight 42 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC004244
Gene symbol BGN
Gene ID (NCBI) 633
RRIDAB_10804656
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Protocols

Product Specific Protocols
WB protocol for Biglycan antibody 16409-1-APDownload protocol
IHC protocol for Biglycan antibody 16409-1-APDownload protocol
IF protocol for Biglycan antibody 16409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncoimmunology

Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer.

Authors - Shaoquan Zheng
humanWB,IF

Cancers (Basel)

Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.

Authors - Kanakaraju Manupati
  • KD Validated
humanWB,IHC

Oncoimmunology

Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.

Authors - Karthikeyan Subbarayan
ratIF

Cell Prolif

Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.

Authors - Yunjiao Wang
humanIHC

Cancer Med

Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma

Authors - Wanyi Xiao
WB

J Proteome Res

Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy

Authors - Yuan Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chronogenic Kit Antigen Retrival ER1 (ph6) for 30 min

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Placenta healthy
Biglycan Antibody Immunohistochemistry validation (1:500 dilution) in Placenta healthy (Cat no:16409-1-AP)