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  • KD/KO Validated

BIN1 Polyclonal antibody

BIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14647-1-AP

Synonyms

AMPH2, Amphiphysin II, Amphiphysin like protein, AMPHL, BIN1, bridging integrator 1, SH3P9



Tested Applications

Positive WB detected inJurkat cells, mouse skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skeletal muscle tissue, human osteosarcoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14647-1-AP targets BIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN1 fusion protein Ag6240
Full Name bridging integrator 1
Calculated molecular weight 65 kDa
Observed molecular weight 50-65 kDa
GenBank accession numberBC004101
Gene symbol BIN1
Gene ID (NCBI) 274
RRIDAB_2243396
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).

Protocols

Product Specific Protocols
WB protocol for BIN1 antibody 14647-1-APDownload protocol
IHC protocol for BIN1 antibody 14647-1-APDownload protocol
IF protocol for BIN1 antibody 14647-1-APDownload protocol
IP protocol for BIN1 antibody 14647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Mol Neurodegener

BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.

Authors - Ari Sudwarts
humanIHC

Int J Cancer

Low expression of Bin1, along with high expression of IDO in tumor tissue and draining lymph nodes, are predictors of poor prognosis for esophageal squamous cell cancer patients.

Authors - Yunlong Jia
humanWB

Mol Biol Cell

Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.

Authors - Giridharan Sai Srinivas Panapakkam SS
  • KD Validated
humanWB

Toxicol Appl Pharmacol

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Authors - Azubuike Peter Ebokaiwe
pigIHC

J Neuropathol Exp Neurol

Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy.

Authors - Jennifer K Lee
pigIHC

J Comp Neurol

Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury.

Authors - Jennifer K Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CaX (Verified Customer) (10-24-2023)

An excellent Ab to probe Bin1 at the right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
BIN1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:14647-1-AP)