Validation Data Gallery
|Positive WB detected in
|Jurkat cells, mouse skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|mouse skeletal muscle tissue, human osteosarcoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse brain tissue
|Western Blot (WB)
|WB : 1:1000-1:6000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14647-1-AP targets BIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, pig
|Host / Isotype
|Rabbit / IgG
|BIN1 fusion protein Ag6240
|bridging integrator 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).
|Product Specific Protocols
|WB protocol for BIN1 antibody 14647-1-AP
|IHC protocol for BIN1 antibody 14647-1-AP
|IF protocol for BIN1 antibody 14647-1-AP
|IP protocol for BIN1 antibody 14647-1-AP
|Click here to view our Standard Protocols
BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.
Int J Cancer
Low expression of Bin1, along with high expression of IDO in tumor tissue and draining lymph nodes, are predictors of poor prognosis for esophageal squamous cell cancer patients.
Mol Biol Cell
Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.
Toxicol Appl Pharmacol
Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.
J Neuropathol Exp Neurol
Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy.
J Comp Neurol
Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
CaX (Verified Customer) (10-24-2023)
An excellent Ab to probe Bin1 at the right MW.