BMI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BMI1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

31

Cat no : 10832-1-AP

Synonyms

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51

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Validation Data Gallery

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  WB analysis of HEK-293 using 10832-1-AP

  WB results of BMI antibody (10832-1-AP, 1:20000) with si-Control and si-BMI transfected HEK293 cells..

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  WB analysis of U-937 using 10832-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10832-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10832-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 10832-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 10832-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 10832-1-AP

  IP Result of anti-BMI1 (IP:10832-1-AP, 4ug; Detection:10832-1-AP 1:500) with U-937 cells lysate 4000ug.

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  IHC staining of human liver cancer using 10832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10832-1-AP

  Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 10832-1-AP

  Immunofluorescent analysis of HepG2 cells, using IL34 antibody 10832-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: U-937 cells, HEK-293 cells, NCCIT cell, mouse brain tissue, K-562 cells
• Positive IP detected in: U-937 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 30 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

10832-1-AP targets BMI1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMI1 fusion protein Ag1286
• Full Name: BMI1 polycomb ring finger oncogene
• Calculated molecular weight: 37 kDa
• Observed molecular weight: 37-43 kDa
• GenBank accession number: BC011652
• Gene symbol: BMI1
• Gene ID (NCBI): 648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Publications

Species	Application	Title
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran ; KD Validated
human	WB	Oncogene; Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.; Authors - Yang Chen
mouse	WB,CoIP,ChIP	J Neurosci; Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.; Authors - Kim Mee-Ohk MO
human	WB	Int J Cancer; SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.; Authors - S-L Xie
human	WB	Oncotarget; Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.; Authors - Xiaolong Wei

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