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  • KD/KO Validated

BMI1 Polyclonal antibody

BMI1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10832-1-AP

Synonyms

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51



Tested Applications

Positive WB detected inU-937 cells, HEK-293 cells, NCCIT cell, mouse brain tissue, K-562 cells
Positive IP detected inU-937 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10832-1-AP targets BMI1 in WB, IP, IHC, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMI1 fusion protein Ag1286
Full Name BMI1 polycomb ring finger oncogene
Calculated molecular weight 37 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC011652
Gene symbol BMI1
Gene ID (NCBI) 648
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Protocols

Product Specific Protocols
WB protocol for BMI1 antibody 10832-1-APDownload protocol
IHC protocol for BMI1 antibody 10832-1-APDownload protocol
IF protocol for BMI1 antibody 10832-1-APDownload protocol
IP protocol for BMI1 antibody 10832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.

Authors - Chenlong Li
humanWB

Cell Cycle

R-spondin1/Wnt-enhanced Ascl2 autoregulation controls the self-renewal of colorectal cancer progenitor cells.

Authors - Jun Ye
humanWB

J Exp Clin Cancer Res

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Authors - Jingyuan Sun
mouseIHC

Med Mol Morphol

A further study on a disturbance of intestinal epithelial cell population and kinetics in APC1638T mice.

Authors - Nami O Wenduerma
mouseWB

Mol Med Rep

CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β‑amyloid metabolism‑associated enzymes.

Authors - Hui Jiang
humanWB,IHC

Int J Med Sci

Downregulation of MiR-203a Disinhibits Bmi1 and Promotes Growth and Proliferation of Keratinocytes in Cholesteatoma.

Authors - Jian Zang
  • KD Validated