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BMI1 Polyclonal antibody

BMI1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 10832-1-AP


BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51

Tested Applications

Positive WB detected inU-937 cells, HEK-293 cells, NCCIT cell, mouse brain tissue, K-562 cells
Positive IP detected inU-937 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10832-1-AP targets BMI1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMI1 fusion protein Ag1286
Full Name BMI1 polycomb ring finger oncogene
Calculated molecular weight 37 kDa
Observed molecular weight37-43 kDa
GenBank accession numberBC011652
Gene symbol BMI1
Gene ID (NCBI) 648
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.


Product Specific Protocols
WB protocol for BMI1 antibody 10832-1-APDownload protocol
IHC protocol for BMI1 antibody 10832-1-APDownload protocol
IF protocol for BMI1 antibody 10832-1-APDownload protocol
IP protocol for BMI1 antibody 10832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Biol Rep

Identification of proline-rich protein 11 as a major regulator in mouse spermatogonia maintenance via an increase in BMI1 protein stability

Authors - Jiajia Xue

Cancer Commun (Lond)

PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c-Myc-mediated positive feedback loop

Authors - Xinyuan Zhao

Med Mol Morphol

A further study on a disturbance of intestinal epithelial cell population and kinetics in APC1638T mice.

Authors - Nami O Wenduerma

Cell Death Dis

Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.

Authors - Chenlong Li

Cell Cycle

R-spondin1/Wnt-enhanced Ascl2 autoregulation controls the self-renewal of colorectal cancer progenitor cells.

Authors - Jun Ye

J Exp Clin Cancer Res

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Authors - Jingyuan Sun