BMI1 Polyclonal antibody

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  WB analysis of HEK-293 using 10832-1-AP

  WB results of BMI antibody (10832-1-AP, 1:20000) with si-Control and si-BMI transfected HEK293 cells..

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  WB analysis using 10832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 10832-1-AP

  IP result of anti-BMI1 (IP:10832-1-AP, 4ug; Detection:10832-1-AP 1:3000) with HT-29 cells lysate 1760 ug.

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  IF Staining of MCF-7 using 10832-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BMI1 antibody (10832-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

BMI1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10832-1-AP

Publications(39)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51

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Tested Applications

• Positive WB detected in: HT-29 cells, HEK-293 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HT-29 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 37 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

10832-1-AP targets BMI1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMI1 fusion protein Ag1286
• Full Name: BMI1 polycomb ring finger oncogene
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37-43 kDa
• GenBank Accession Number: BC011652
• Gene Symbol: BMI1
• Gene ID (NCBI): 648
• RRID: AB_2065392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35226
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Publications

Species	Application	Title
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran ; KD Validated
human	WB	Oncogene; Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.; Authors - Yang Chen
mouse	WB,CoIP,ChIP	J Neurosci; Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.; Authors - Kim Mee-Ohk MO
human	WB	Int J Cancer; SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.; Authors - S-L Xie
human	WB	Oncotarget; Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.; Authors - Xiaolong Wei