BMP2 Monoclonal antibody

BMP2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (2)

Applications

WB, IHC, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

26

CloneNo.

1C5C2

Cat no : 66383-1-Ig

Synonyms

BMP 2, BMP 2A, BMP2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

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Validation Data Gallery

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  WB analysis of pig bone marrow using 66383-1-Ig

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66383-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66383-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: pig bone marrow tissue, Caco-2 cells, HeLa cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 24 publications below
• IHC: See 3 publications below

Product Information

66383-1-Ig targets BMP2 in WB, IHC, Cell treatment, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: BMP2 fusion protein Ag13501
• Full Name: bone morphogenetic protein 2
• Calculated molecular weight: 396 aa, 44 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC069214
• Gene symbol: BMP2
• Gene ID (NCBI): 650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.

Publications

Species	Application	Title
human	WB	Angiogenesis; Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8.; Authors - Vivien Becker
human	WB	Oncogenesis; Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.; Authors - Lin Lu
human	WB	Front Oncol; Increased Methyl-CpG-Binding Domain Protein 2 Promotes Cigarette Smoke-Induced Pulmonary Hypertension.; Authors - Jixing Wu
rat	WB	J Hypertens; Saturated fatty acid promotes calcification via suppressing SIRT6 expression in vascular smooth muscle cells; Authors - Yafen Tao
human,mouse	Cell treatment	J Cell Mol Med; STARD3NL inhibits the osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2 in osteoporosis.; Authors - Yuexin Xu
mouse	WB,IHC	Front Pharmacol; Yi-Shen-Hua-Shi Granule Alleviates Adriamycin-Induced Glomerular Fibrosis by Suppressing the BMP2/Smad Signaling Pathway.; Authors - Zhuojing Tan