BMP2 Monoclonal antibody

BMP2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (2)

Applications

WB, IHC, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

1C5C2

Cat no : 66383-1-Ig

Synonyms

BMP 2, BMP 2A, BMP2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A



Tested Applications

Positive WB detected inpig bone marrow tissue, Caco-2 cells, HeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66383-1-Ig targets BMP2 in WB, IHC, Cell treatment, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BMP2 fusion protein Ag13501
Full Name bone morphogenetic protein 2
Calculated molecular weight 396 aa, 44 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC069214
Gene symbol BMP2
Gene ID (NCBI) 650
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.

Protocols

Product Specific Protocols
WB protocol for BMP2 antibody 66383-1-IgDownload protocol
IHC protocol for BMP2 antibody 66383-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Angiogenesis

Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8.

Authors - Vivien Becker
humanWB

Oncogenesis

Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.

Authors - Lin Lu
humanWB

Front Oncol

Increased Methyl-CpG-Binding Domain Protein 2 Promotes Cigarette Smoke-Induced Pulmonary Hypertension.

Authors - Jixing Wu
ratWB

J Hypertens

Saturated fatty acid promotes calcification via suppressing SIRT6 expression in vascular smooth muscle cells

Authors - Yafen Tao
human,mouseCell treatment

J Cell Mol Med

STARD3NL inhibits the osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2 in osteoporosis.

Authors - Yuexin Xu
mouseWB,IHC

Front Pharmacol

Yi-Shen-Hua-Shi Granule Alleviates Adriamycin-Induced Glomerular Fibrosis by Suppressing the BMP2/Smad Signaling Pathway.

Authors - Zhuojing Tan