Tested Applications
| Positive WB detected in | pig bone marrow tissue, Caco-2 cells, HeLa cells, pig aorta tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66383-1-Ig targets BMP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13501 Product name: Recombinant human BMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC069214 Sequence: MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYRRHSGQPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQVFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTSLLDTRLVNQNASRWESFDVTPAVMRWTAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKRQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR Predict reactive species |
| Full Name | bone morphogenetic protein 2 |
| Calculated Molecular Weight | 396 aa, 44 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC069214 |
| Gene Symbol | BMP2 |
| Gene ID (NCBI) | 650 |
| RRID | AB_2881759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12643 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| WB protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BMP2 Monoclonal antibody (1C5C2), catalog number 66383-1-Ig, has accumulated 118 citations across journals including Nature Communications, Cancer Cell, Theranostics, Advanced Science, ACS Applied Materials & Interfaces, Stem Cell Research & Therapy, Phytomedicine, Cell Death & Disease, and FASEB Journal, among others. Research fields span bone regeneration/osteogenesis, vascular calcification, osteoporosis, cancer biology, biomaterials engineering, periodontal/dental research, and metabolic/endocrine disorders.
| Species | Application | Title |
|---|---|---|
Cancer Cell Tumor cell-intrinsic epigenetic dysregulation shapes cancer-associated fibroblasts heterogeneity to metabolically support pancreatic cancer | ||
Ann Rheum Dis Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration | ||
Bioact Mater Continuously released Zn2+ in 3D-printed PLGA/β-TCP/Zn scaffolds for bone defect repair by improving osteoinductive and anti-inflammatory properties | ||
Nat Commun Injectable hydrogels for osteomyelitis treatment induce metabolic reprogramming for protection against reinfection. | ||
J Adv Res Genomic insights into the population history of fat-tailed sheep and identification of two mutations that contribute to fat tail adipogenesis | ||
Redox Biol Small molecule HCY-NBD stabilizes GSTM2 via cys174 sulfenylation to attenuate high glucose induced endothelial cell senescence and calcification. |
