BMP2 Monoclonal antibody
BMP2 Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig and More (2)
Applications
WB, IHC, IF, Cell treatment, ELISA
Conjugate
Unconjugated
CloneNo.
1C5C2
Cat no : 66383-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig bone marrow tissue, Caco-2 cells, HeLa cells |
Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 27 publications below |
IHC | See 4 publications below |
IF | See 2 publications below |
Product Information
66383-1-Ig targets BMP2 in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | BMP2 fusion protein Ag13501 |
Full Name | bone morphogenetic protein 2 |
Calculated molecular weight | 396 aa, 44 kDa |
Observed molecular weight | 45 kDa |
GenBank accession number | BC069214 |
Gene symbol | BMP2 |
Gene ID (NCBI) | 650 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.
Protocols
Product Specific Protocols | |
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WB protocol for BMP2 antibody 66383-1-Ig | Download protocol |
IHC protocol for BMP2 antibody 66383-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Appl Mater Interfaces Nitrogen-Doped Multiwalled Carbon Nanotubes Enhance Bone Remodeling through Immunomodulatory Functions. | ||
Angiogenesis Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8. | ||
Phytother Res Bavachinin selectively modulates PPAR γ and maintains bone homeostasis in Type 2 Diabetes | ||
Oncogenesis Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway. | ||
J Hypertens Saturated fatty acid promotes calcification via suppressing SIRT6 expression in vascular smooth muscle cells | ||
Front Oncol Increased Methyl-CpG-Binding Domain Protein 2 Promotes Cigarette Smoke-Induced Pulmonary Hypertension. |