BMP2K Polyclonal antibody

• 

  WB analysis using 32853-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32853-1-AP (BMP2K antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

BMP2K Polyclonal Antibody for WB, ELISA

Cat No. 32853-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BIKe, BMP-2-inducible protein kinase, EC:2.7.11.1, HRIHFB2017

---

Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32853-1-AP targets BMP2K in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMP2K fusion protein Ag36482
• Full Name: BMP2 inducible kinase
• Calculated Molecular Weight: 129kDa，1161aa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_198892.1
• Gene Symbol: BMP2K
• Gene ID (NCBI): 55589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NSY1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMP2K is an uncharacterized member of NAK family. Structural similarities of kinase domains (75% identical) and in vitro inhibition profiles categorized BMP2K as the closest homolog of AAK1. BMP2K was originally identified as a serine-threonine kinase regulating osteoblast differentiation whose gene product is sharply upregulated by BMP-2 stimulation. Later, various genetic and proteomic screens linked BMP2K to HIV entry, developmental dysplasia of the hip, myopia, leukemia, breast cancer metastasis and so on. Recent multivariate proteomic profiling approaches identified BMP2K as a component of purified CCV from HeLa cells. BMP2K can phosphorylate AP-2 in vitro and in vivo. Functional impairment of BMP2K impedes AP-2 phosphorylation leading to defects in clathrin-coated pit (CCP) morphology and cargo internalization. BMP2K engages AP-2 via its extended C-terminus and this interaction is important for its CCP localization and function (PMID: 34480404).