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BRCA1 Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 20649-1-AP

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Con: 58 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

BRCA1 Antibody WB human testis tissue 20649-1-AP

human testis tissue were subjected to SDS PAGE followed by western blot with 20649-1-AP(BRCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

BRCA1 Antibody IHC human breast cancer tissue 20649-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 20649-1-AP(BRCA1 antibody) at dilution of 1:100 (under 40x lens)

BRCA1 Antibody IP mouse testis tissue 20649-1-AP

IP Result of anti-BRCA1 (IP:20649-1-AP, 4ug; Detection:20649-1-AP 1:500) with mouse testis tissue lysate 4800ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
human testis tissue, A2780 cells, A431 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected in:
mouse testis tissue
Positive IHC detected in:
human breast cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
breast cancer 1, early onset
Calculated molecular weight:
208 kDa
Observed molecular weight:
85-95 kDa
GenBank accession number:
NM_007294
Gene ID (NCBI):
672
Gene symbol
BRCA1
Synonyms
BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53

Background

RCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.


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