BSAP,PAX5 Polyclonal antibody

• Featured Product
• KD/KO Validated

BSAP,PAX5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 26709-1-AP

Synonyms

PAX5, PAX 5, Paired box protein Pax-5, Paired box protein Pax 5, paired box 5

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Validation Data Gallery

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  WB analysis using 26709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 26709-1-AP

  WB result of BSAP,PAX5 antibody (26709-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BSAP,PAX5 transfected Raji cells.

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  WB analysis of Ramos using 26709-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 26709-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Ramos using 26709-1-AP

  IP result of anti-BSAP,PAX5 (IP:26709-1-AP, 4ug; Detection:26709-1-AP 1:1000) with Ramos cells lysate 3600 ug.

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  IHC staining of human tonsillitis using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IP detected in: Ramos cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Product Information

26709-1-AP targets BSAP,PAX5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BSAP,PAX5 fusion protein Ag25147
• Full Name: paired box 5
• Calculated Molecular Weight: 391 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC156927
• Gene Symbol: PAX5
• Gene ID (NCBI): 5079
• RRID: AB_2880608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

Publications

Species	Application	Title
human	IHC	Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
human	IHC	J Cutan Pathol; Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases.; Authors - Jennifer S Ko
mouse	IHC	Mol Oncol; miR-126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis-associated colorectal cancer; Authors - Shuai Wu
human	WB,IP	Free Radic Biol Med; Prohibitin 2 confers NADPH oxidase 1 -mediated cytosolic oxidative signaling to promote gastric cancer progression by ERK activation; Authors - Liang Xu
mouse	WB	Cell Mol Immunol; Rack1 regulates B-cell development and function by binding to and stabilizing the transcription factor Pax5; Authors - Xueting Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-28-2024)

This antibody was used to detect Pax5 positive cells in tonsil and worked well for immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Tonsil