|Positive WB detected in||human liver tissue, Jurkat cells, mouse liver tissue, mouse heart tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11989-1-AP targets CD147 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD147 fusion protein Ag2619|
|Full Name||basigin (Ok blood group)|
|Calculated molecular weight||385 aa, 42 kDa|
|Observed molecular weight||42-50 kDa|
|GenBank accession number||BC009040|
|Gene ID (NCBI)||682|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BSG, also named as 5F7, Basigin, EMMPRIN, TCSF and CD147, plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA, inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix, plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.
J Physiol Biochem
α2,6-Sialylation promotes immune escape in hepatocarcinoma cells by regulating T cell functions and CD147/MMP signaling.
The fucosylated CD147 enhances the autophagy in epithelial ovarian cancer cells.
Naunyn Schmiedebergs Arch Pharmacol
LncRNA RHPN1-AS1 accelerates proliferation, migration, and invasion via regulating miR-485-5p/BSG axis in hepatocellular carcinoma.
Triptolide inhibits the migration and invasion of human prostate cancer cells via Caveolin-1/CD147/MMPs pathway.
Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma.
Effects of excess thromboxane A2 on placental development and nutrient transporters in a Mus musculus model of fetal growth restriction.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mohammed (Verified Customer) (08-19-2020)
Stains the sperm tail very nicely.