CD147 Polyclonal antibody

CD147 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11989-1-AP

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF



Tested Applications

Positive WB detected inhuman liver tissue, Jurkat cells, mouse liver tissue, mouse heart tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11989-1-AP targets CD147 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD147 fusion protein Ag2619
Full Name basigin (Ok blood group)
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC009040
Gene symbol BSG
Gene ID (NCBI) 682
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BSG, also named as 5F7, Basigin, EMMPRIN, TCSF and CD147, plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA, inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix, plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.

Protocols

Product Specific Protocols
WB protocol for CD147 antibody 11989-1-APDownload protocol
IHC protocol for CD147 antibody 11989-1-APDownload protocol
IP protocol for CD147 antibody 11989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Physiol Biochem

α2,6-Sialylation promotes immune escape in hepatocarcinoma cells by regulating T cell functions and CD147/MMP signaling.

Authors - Liping Wang
IP

Oncotarget

The fucosylated CD147 enhances the autophagy in epithelial ovarian cancer cells.

Authors - Zhenhua Hu
humanWB

Naunyn Schmiedebergs Arch Pharmacol

LncRNA RHPN1-AS1 accelerates proliferation, migration, and invasion via regulating miR-485-5p/BSG axis in hepatocellular carcinoma.

Authors - Wei Zhang
humanWB

Biomed Pharmacother

Triptolide inhibits the migration and invasion of human prostate cancer cells via Caveolin-1/CD147/MMPs pathway.

Authors - Shiqi Yuan
humanIHC

Histopathology

Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma.

Authors - Huang Congfa C

Biol Reprod

Effects of excess thromboxane A2 on placental development and nutrient transporters in a Mus musculus model of fetal growth restriction.

Authors - Karen J Gibbins

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mohammed (Verified Customer) (08-19-2020)

Stains the sperm tail very nicely.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: (1:200)
  • Cell Tissue Type: Testis