Tested Applications
| Positive WB detected in | HeLa cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NCCIT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13560-1-AP targets BST2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4430 Product name: Recombinant human BST2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC033873 Sequence: MASTSYDYCRVPMEDGDKRCKLLLGIGILVLLIIVILGVPLIIFTIKANSEACRDGLRAVMECRNVTHLLQQELTEAQKGFQDVEAQAATCNHTVMALMASLDAEKAQGQKKVEELEGEITTLNHKLQDASAEVERLRRENQVLSVRIADKKYYPSSQDSSSAAAPQLLIVLLGLSALLQ Predict reactive species |
| Full Name | bone marrow stromal cell antigen 2 |
| Calculated Molecular Weight | 180 aa, 20 kDa |
| Observed Molecular Weight | 30-36 kDa, 50-70 kDa |
| GenBank Accession Number | BC033873 |
| Gene Symbol | BST2 |
| Gene ID (NCBI) | 684 |
| RRID | AB_2067220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q10589 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks retrovirus release from the cell surface. Depleted upon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane protein, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 are 30-36 kDa (monomer form) and 50-70 kDa (dimer form) (PMID: 19196977; 21237475; 19737401; 37922253).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BST2 antibody 13560-1-AP | Download protocol |
| IHC protocol for BST2 antibody 13560-1-AP | Download protocol |
| IP protocol for BST2 antibody 13560-1-AP | Download protocol |
| WB protocol for BST2 antibody 13560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BST2/Tetherin antibody (catalog #13560-1-AP) has accumulated 36 citations published in journals including Nature Communications, Molecular Cell, PNAS, EMBO Molecular Medicine, Cell Reports, Current Biology, Journal of Virology, PLoS Pathogens, and eLife. Research fields associated with these citations include virology (HIV, HBV, HCV, SARS-CoV-2, dengue), oncology (lung, gastric, pancreatic, esophageal, and thyroid cancers), innate immunity, type I interferon signaling, autophagy, and cellular proteostasis.
| Species | Application | Title |
|---|---|---|
Nat Commun Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef | ||
Cell Death Dis CD317 maintains proteostasis and cell survival in response to proteasome inhibitors by targeting calnexin for RACK1-mediated autophagic degradation
| ||
EMBO Mol Med RADX protects against intestinal inflammation by restraining IFI16-mediated innate immunity. | ||
Curr Biol The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.
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Reviews
What customers say
All three reviews are 5-star for Western Blot at a 1:1000 dilution. Users report reliable detection of endogenous BST-2 in HeLa cells, including both dimers and monomers. Specificity is confirmed by the absence of signal in BST-2-negative HEK293T cells, while overexpressed FLAG-tagged or untagged BST-2 is clearly detected. One user noted that dry transfer failed but classic wet transfer with 20% ethanol worked well. Overall, reviewers find the antibody efficient and specific for its intended target.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sen (Verified Customer) (04-16-2025) | The BST-2 protein could not be detected when I do the dry transfer, I don't know why. However, when I do the classic transfer with 20% of ethanol, it works.
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Margaux (Verified Customer) (06-13-2024) | Very good efficiency. In HeLa cells, I detected BST2 dimers and monomers at the dilution 1/1000.
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Christine (Verified Customer) (10-08-2021) | Detects endogenous BST2 in HeLa cells (BST2 positive), also when overexpressed as FLAG tagged version in HeLa cells. Does no detect anything in HEK293T cells (BST2 negative) but detects many bands when FLAG BST2 or untagged BST2 is overexpressed in these cells.
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