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  • KD/KO Validated

BST2 Polyclonal antibody

BST2 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 13560-1-AP


Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin

Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13560-1-AP targets BST2 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BST2 fusion protein Ag4430
Full Name bone marrow stromal cell antigen 2
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 30-36 kDa, 50-70 kDa
GenBank Accession NumberBC033873
Gene Symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).


Product Specific Protocols
WB protocol for BST2 antibody 13560-1-APDownload protocol
IHC protocol for BST2 antibody 13560-1-APDownload protocol
IF protocol for BST2 antibody 13560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

PMID: 28965816

Authors - Shouheng Jin
  • KD Validated

Proc Natl Acad Sci U S A

PMID: 24753610

Authors - Xuesen Zhao
humanWB, IF

Curr Biol

The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.

Authors - Adrien Presle
  • KO Validated

PLoS Pathog

PMID: 28910400

Authors - Yuxuan Fu
  • KO Validated
humanWB, IF

J Virol

Interferon-inducible Protein Tetherin Inhibits Hepatitis B Virus Virion Secretion.

Authors - Ran Yan
  • KD Validated

J Virol

Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms.

Authors - Weidner Jessica M JM


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Christine (Verified Customer) (10-08-2021)

Detects endogenous BST2 in HeLa cells (BST2 positive), also when overexpressed as FLAG tagged version in HeLa cells. Does no detect anything in HEK293T cells (BST2 negative) but detects many bands when FLAG BST2 or untagged BST2 is overexpressed in these cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cell culture of HeLa and HEK293T