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  • KD/KO Validated

BST2 Polyclonal antibody

BST2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13560-1-AP

Synonyms

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin



Tested Applications

Positive WB detected inhuman heart tissue, human liver tissue, L02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13560-1-AP targets BST2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BST2 fusion protein Ag4430
Full Name bone marrow stromal cell antigen 2
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 30-36 kDa
GenBank accession numberBC033873
Gene symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Protocols

Product Specific Protocols
WB protocol for BST2 antibody 13560-1-APDownload protocol
IHC protocol for BST2 antibody 13560-1-APDownload protocol
IF protocol for BST2 antibody 13560-1-APDownload protocol
FC protocol for BST2 antibody 13560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biotechnol Lett

BST2 promotes cell proliferation, migration and induces NF-κB activation in gastric cancer.

Authors - Weiyu Liu
  • KD Validated
humanWB

J Biochem

MicroRNA-760 inhibits cell viability and migration through down-regulating BST2 in gastric cancer.

Authors - Weiyu Liu
humanWB

Biochem Biophys Res Commun

PMID: 24735544

Authors - Wei Zhang
humanWB,IF

PLoS One

BST2/tetherin inhibits dengue virus release from human hepatoma cells.

Authors - Pan Xiao-Ben XB
humanWB,IF

Antiviral Res

BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells.

Authors - Pan Xiao-Ben XB
humanFC

J Virol

Preadaptation of SIVsmm facilitated Env-mediated counteraction of human tetherin by HIV-2.

Authors - Elena Heusinger