- Featured Product
- KD/KO Validated
Bcl-XL Polyclonal antibody
Bcl-XL Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10783-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, Raji cells, mouse placenta tissue, HepG2 cells, C6 cells, Jurkat cells, K-562 cells, human placenta tissue, NIH/3T3 cells, RAW264.7, HeLa cells, rat brain tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human lung cancer tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10783-1-AP targets Bcl-XL in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||goat, human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bcl-XL fusion protein Ag1232|
|Full Name||BCL2-like 1|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC019307|
|Gene ID (NCBI)||598|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta) , and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).
|Product Specific Protocols|
|WB protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|IHC protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|IF protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|IP protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|FC protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Glutaminolysis Mediated by MALT1 Protease Activity Facilitates PD-L1 Expression on ABC-DLBCL Cells and Contributes to Their Immune Evasion.
Biochem Biophys Res Commun
Long non-coding RNA FAM84B-AS promotes resistance of gastric cancer to platinum drugs through inhibition of FAM84B expression.
Cell Biol Int
Soluble CXCL16 Promotes TNF-α-induced Apoptosis in DLBCL via the AMAD10-NF-κB Regulatory Feedback Loop.
A novel hydrogen sulfide-releasing donor, HA-ADT, suppresses the growth of human breast cancer cells through inhibiting the PI3K/AKT/mTOR and Ras/Raf/MEK/ERK signaling pathways.
Biochem Biophys Res Commun
Alisol B-23-acetate, a tetracyclic triterpenoid isolated from Alisma orientale, induces apoptosis in human lung cancer cells via the mitochondrial pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Bastien (Verified Customer) (08-19-2020)
Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody