Featured Product
KD/KO Validated

BCL2L1 Polyclonal antibody

BCL2L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10783-1-AP

Publications (252)

Reviews (5)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

Bcl-xL, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1, Bcl-2-like protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis using 10783-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa cells using 10783-1-AP
WB result of Bcl-XL antibody (10783-1-AP, 1:800) with si-Control and si-Bcl-XL transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Caco-2 using 10783-1-AP
WB result of BCL2L1 antibody (10783-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL2L1 transfected Caco-2 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of C6 using 10783-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NIH/3T3 using 10783-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Raji using 10783-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 10783-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse placenta using 10783-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of RAW264.7 using 10783-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human placenta using 10783-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 10783-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 10783-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat brain using 10783-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of k-562 using 10783-1-AP
IP result of anti-Bcl-XL (IP:10783-1-AP, 4ug; Detection:10783-1-AP 1:1000) with k-562 cells lysate 1760 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of NIH/3T3 using 10783-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl-XL antibody (10783-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for BCL2L1 in ?

Overview

Tested Applications
Product Information
Publications (252)
Protocols
Reviews (5)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, C6 cells, Caco-2 cells, HeLa cells, human placenta tissue, Jurkat cells, K-562 cells, mouse placenta tissue, NIH/3T3 cells, Raji cells, rat brain tissue, RAW264.7
Positive IP detected in	k-562 cells
Positive IHC detected in	human lung cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10783-1-AP targets BCL2L1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, zebrafish, hamster, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1232 Product name: Recombinant human Bcl-xL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-233 aa of BC019307 Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK Predict reactive species
Full Name	BCL2-like 1
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC019307
Gene Symbol	BCL2L1
Gene ID (NCBI)	598
RRID	AB_2064724
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07817
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK, or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Protocols

Product Specific Protocols
IF protocol for BCL2L1 antibody 10783-1-AP	Download protocol
IHC protocol for BCL2L1 antibody 10783-1-AP	Download protocol
IP protocol for BCL2L1 antibody 10783-1-AP	Download protocol
WB protocol for BCL2L1 antibody 10783-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

10783-1-AP is an anti-Bcl-xL (BCL2L1) antibody with 252 total citations. Citations span over 80 journals, including Immunity, Nature Communications, Cancer Research, Cell Death & Differentiation, Cell Death & Disease, Oncogene, Free Radical Biology and Medicine, and Biomaterials. Research fields cover apoptosis, ferroptosis, mitochondrial biology, and diverse cancer types (hepatocellular, lung, breast, colorectal, pancreatic), as well as drug development, neuroprotection, and inflammation. Western blot is the predominant application.

Species	Application	Title
		Immunity cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity Authors - Yunlong Zhao View Article
mouse,human	WB	Cancer Res Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer. Authors - Yogev Sela View Article
human	WB	Nat Commun Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction. Authors - Tian-Ying Zhang View Article
human	WB	J Nanobiotechnology ROS-responsive EGCG-Fe-Cur nanosystems for radioprotection through antioxidant and inhibition of ferroptosis pathways. Authors - Xinrui Yang View Article
human	WB	Acta Pharm Sin B Avenanthramide A potentiates Bim-mediated antineoplastic properties of 5-fluorouracil via targeting KDM4C/MIR17HG/GSK-3β negative feedback loop in colorectal cancer Authors - Rong Fu View Article
human	WB	J Exp Clin Cancer Res ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer. Authors - Shuohui Dong View Article

Reviews

4.2 out of 5

Add a review

5 verified customer reviews

What customers say

Users report strong, clear bands for BCL-XL detection in Western Blot, particularly in cancer cell lines, with optimal dilutions ranging from 1:800 to 1:2000. The antibody is described as easy to work with and effective across multiple applications including IHC, IF, and flow cytometry. One reviewer noted a few nonspecific bands above 37 kD when detecting endogenous BCL2L1 at 30 kD. Overall, the product receives favorable ratings (average ~4.2/5), with users highlighting its reliability and role in successful research projects.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Mounika (Verified Customer) (03-24-2026)	very easy antibody to work with and played important role in research project! Applications: Western Blot, Immunohistochemistry, Immunofluorescence Primary Antibody Dilution: 1:800
Sai Sindhura (Verified Customer) (01-08-2026)	BCL-XL gives very good bands Applications: Western Blot, Other Primary Antibody Dilution: 1:1000
N (Verified Customer) (11-04-2025)	This Ab is okay to detect endo BCL2L1 (30 kD), although there are a few nonspecific bands above 37 kD. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: PC12 cell lysate
Shinford (Verified Customer) (10-26-2023)	In cancer cell lines, the bands appeared significantly clearer, and the optimal concentration was found to be 1:1000. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Lung cancer cell lines BCL2L1 Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:10783-1-AP)
Bastien (Verified Customer) (08-19-2020)	Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody Applications: Flow Cytometry Primary Antibody Dilution: 0,05µl/10^6 cells Cell Tissue Type: B cells BCL2L1 Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:10783-1-AP)