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  • KD/KO Validated

Bcl-XL Polyclonal antibody

Bcl-XL Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10783-1-AP

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL



Tested Applications

Positive WB detected inA549 cells, Raji cells, Jurkat cells, rat brain tissue, HeLa cells, NIH/3T3 cells, human placenta tissue, C6 cells, K-562 cells, mouse placenta tissue, RAW264.7
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10783-1-AP targets Bcl-XL in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, hamster, sheep, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag1232
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta) , and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 10783-1-APDownload protocol
IHC protocol for Bcl-XL antibody 10783-1-APDownload protocol
IF protocol for Bcl-XL antibody 10783-1-APDownload protocol
IP protocol for Bcl-XL antibody 10783-1-APDownload protocol
FC protocol for Bcl-XL antibody 10783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction.

Authors - Tian-Ying Zhang
human,mouseWB

Cancer Res

Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.

Authors - Yogev Sela
humanWB

Oncotarget

Overexpressed EDIL3 predicts poor prognosis and promotes anchorage-independent tumor growth in human pancreatic cancer.

Authors - Shu-Heng Jiang
humanWB

Oncotarget

By reducing hexokinase 2, resveratrol induces apoptosis in HCC cells addicted to aerobic glycolysis and inhibits tumor growth in mice.

Authors - Weiqi Dai
humanWB

Int J Cancer

Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.

Authors - Liu Chaoyang C
mouseWB

J Bone Miner Res

Sclerostin mediates bone response to mechanical unloading through antagonizing Wnt/beta-catenin signaling.

Authors - Lin Chuwen C

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0,05µl/10^6 cells
  • Cell Tissue Type: B cells
Bcl-XL Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:10783-1-AP)