Bcl2 Monoclonal antibody

Bcl2 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse and More (1)

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66799-1-Ig

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik



Tested Applications

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells, mouse brain tissue, C2C12 cells, 4T1 cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66799-1-Ig targets Bcl2 in WB, IP, IF,ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bcl2 fusion protein Ag24261
Full Name B-cell leukemia/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberNM-009741
Gene symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 66799-1-IgDownload protocol
IF protocol for Bcl2 antibody 66799-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pharmazie

Anti-CD19 mAb-conjugated human serum albumin nanoparticles effectively deliver doxorubicin to B-lymphoblastic leukemia cells.

Authors - H Pan
mouseWB

Biochem Biophys Res Commun

Hyper-O-GlcNAcylation impairs insulin response against reperfusion-induced myocardial injury and arrhythmias in obesity.

Authors - Lingyan Jin
humanWB

J Nat Prod

Dual-Targeting Antiproliferation Hybrids Derived from 1-Deoxynojirimycin and Kaempferol Induce MCF-7 Cell Apoptosis through the Mitochondria-Mediated Pathway.

Authors - Ran Zhang
mouseWB

Food Funct

Camellia oil (Camellia oleifera Abel.) attenuates CCl4-induced liver fibrosis via suppressing hepatocyte apoptosis in mice.

Authors - Xiaohua Lei
mouseWB, IP

Cell Prolif

Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.

Authors - Li Chen

Redox Biol

Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload.

Authors - Chen Chen