Bcl2 Monoclonal antibody

Bcl2 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse and More (2)

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66799-1-Ig

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik



Tested Applications

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells, mouse brain tissue, C2C12 cells, 4T1 cells
Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66799-1-Ig targets Bcl2 in WB, IP, IF, FC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bcl2 fusion protein Ag24261
Full Name B-cell leukemia/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberNM-009741
Gene symbol Bcl2
Gene ID (NCBI) 12043
RRIDAB_2882142
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 66799-1-IgDownload protocol
IF protocol for Bcl2 antibody 66799-1-IgDownload protocol
FC protocol for Bcl2 antibody 66799-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

Puerarin prevents sepsis-associated encephalopathy by regulating the AKT1 pathway in microglia

Authors - Shao-Peng Lin
human,mouseWB

Biomed Pharmacother

Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice

Authors - Xiao-Hong Xiang

Redox Biol

Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload.

Authors - Chen Chen
mouseWB

Antioxidants (Basel)

Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress

Authors - Wenji Ren
mouseWB, IP

Cell Prolif

Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.

Authors - Li Chen
mouseWB

Int J Mol Sci

Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses

Authors - Jin Sun