mouse Bcl2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Bcl2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

71

CloneNo.

1B3F7

Reviews (1)

Cat no : 68103-1-Ig

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik

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Validation Data Gallery

• 

  WB analysis using 68103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 68103-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 68103-1-Ig

  C2C12 cells were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 68103-1-Ig

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 68103-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HSC-T6 cells, C2C12 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue, rat spleen tissue, RAW 264.7 cells, rat brain tissue
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 68 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

68103-1-Ig targets Bcl2 in WB, IF, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Bcl2 fusion protein Ag24261
• Full Name: B-cell leukemia/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM-009741
• Gene Symbol: Bcl2
• Gene ID (NCBI): 12043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Publications

Species	Application	Title
	WB	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
mouse	WB	Bioact Mater; Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma; Authors - Yuhan Wang
human,mouse	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
mouse	WB	J Nanobiotechnology; Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome; Authors - Sisi Yan
human	WB	Front Immunol; A programmed cell death-related model based on machine learning for predicting prognosis and immunotherapy responses in patients with lung adenocarcinoma; Authors - Yi Zhang
mouse	WB	Ecotoxicol Environ Saf; Cresyl Diphenyl Phosphate exposure induces reproductive functional defects in men and male mice; Authors - Danni Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Amandine (Verified Customer) (02-07-2024)

20ug of protein denatured or not, primary antibody 1/5000 RT and secondary antibody 1/5000 2h RT. Chemiluminescence revelation with 10min of exposition

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: ImCC

Bcl2 Antibody Western Blot validation (1/5000 dilution) in ImCC (Cat no:68103-1-Ig)