Bcl2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Bcl2 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

CloneNo.

1B3F7

Cat no : 68103-1-Ig

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik

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Validation Data Gallery

• 

  WB analysis using 68103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68103-1-Ig

  Jurkat and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and blotted with Caspase 3 antibody followed by re-blotting with beta actin antibody as loading control.

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  WB analysis of mouse brain using 68103-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 68103-1-Ig

  WB result of Bcl2 antibody (68103-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Bcl2 transfected Jurkat cells.

• 

  WB analysis using 68103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 68103-1-Ig

  C2C12 cells were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 68103-1-Ig

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 68103-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, C2C12 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue, Jurkat cells, HEK-293 cells, HSC-T6 cells, rat spleen tissue, RAW 264.7 cells, rat brain tissue
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 16 publications below
• IHC: See 3 publications below

Product Information

68103-1-Ig targets Bcl2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Bcl2 fusion protein Ag24261
• Full Name: B-cell leukemia/lymphoma 2
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 26 kDa
• GenBank accession number: NM-009741
• Gene symbol: Bcl2
• Gene ID (NCBI): 12043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Publications

Species	Application	Title
human	WB	Phytother Res; Renal transporter OAT1 and PPAR-α pathway co-contribute to icaritin-induced nephrotoxicity; Authors - Dalong Wang
mouse	WB	Small; Multifunctional Silk Fibroin Methacryloyl Microneedle for Diabetic Wound Healing; Authors - Gaopeng Guan
rat	WB	Nutrients; Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats; Authors - Yujie Zhong
rat	WB	Cell Stress Chaperones; Elabela blunts doxorubicin-induced oxidative stress and ferroptosis in rat aortic adventitial fibroblasts by activating the KLF15/GPX4 signaling; Authors - Mi-Wen Zhang
human	WB	Toxicon; MiR-214-3p may alleviate T-2 toxin-induced chondrocyte apoptosis and matrix degradation by regulating NF-κB signaling pathway in vitro; Authors - Lele Liu
human	WB,IHC	Front Pharmacol; Promotion of colorectal cancer cell death by ezetimibe via mTOR signaling-dependent mitochondrial dysfunction; Authors - Yuanyuan Zheng