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Bcl2 Monoclonal antibody
Bcl2 Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1B3F7
Cat no : 68103-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, C2C12 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue, Jurkat cells, HEK-293 cells, HSC-T6 cells, rat spleen tissue, RAW 264.7 cells, rat brain tissue |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 16 publications below |
IHC | See 3 publications below |
Product Information
68103-1-Ig targets Bcl2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Bcl2 fusion protein Ag24261 |
Full Name | B-cell leukemia/lymphoma 2 |
Calculated molecular weight | 26 kDa |
Observed molecular weight | 26 kDa |
GenBank accession number | NM-009741 |
Gene symbol | Bcl2 |
Gene ID (NCBI) | 12043 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Bcl2 antibody 68103-1-Ig | Download protocol |
IF protocol for Bcl2 antibody 68103-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Phytother Res Renal transporter OAT1 and PPAR-α pathway co-contribute to icaritin-induced nephrotoxicity | ||
Nutrients Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats | ||
Cell Stress Chaperones Elabela blunts doxorubicin-induced oxidative stress and ferroptosis in rat aortic adventitial fibroblasts by activating the KLF15/GPX4 signaling | ||
Toxicon MiR-214-3p may alleviate T-2 toxin-induced chondrocyte apoptosis and matrix degradation by regulating NF-κB signaling pathway in vitro | ||
Front Pharmacol Promotion of colorectal cancer cell death by ezetimibe via mTOR signaling-dependent mitochondrial dysfunction |