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Bcl2 Monoclonal antibody

Bcl2 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B3F7

Cat no : 68103-1-Ig

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik



Tested Applications

Positive WB detected inHeLa cells, C2C12 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue, Jurkat cells, HEK-293 cells, HSC-T6 cells, rat spleen tissue, RAW 264.7 cells, rat brain tissue
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68103-1-Ig targets Bcl2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bcl2 fusion protein Ag24261
Full Name B-cell leukemia/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberNM-009741
Gene symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 68103-1-IgDownload protocol
IF protocol for Bcl2 antibody 68103-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Phytother Res

Renal transporter OAT1 and PPAR-α pathway co-contribute to icaritin-induced nephrotoxicity

Authors - Dalong Wang
mouseWB

Small

Multifunctional Silk Fibroin Methacryloyl Microneedle for Diabetic Wound Healing

Authors - Gaopeng Guan
ratWB

Nutrients

Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats

Authors - Yujie Zhong
ratWB

Cell Stress Chaperones

Elabela blunts doxorubicin-induced oxidative stress and ferroptosis in rat aortic adventitial fibroblasts by activating the KLF15/GPX4 signaling

Authors - Mi-Wen Zhang
humanWB

Toxicon

MiR-214-3p may alleviate T-2 toxin-induced chondrocyte apoptosis and matrix degradation by regulating NF-κB signaling pathway in vitro

Authors - Lele Liu
humanWB,IHC

Front Pharmacol

Promotion of colorectal cancer cell death by ezetimibe via mTOR signaling-dependent mitochondrial dysfunction

Authors - Yuanyuan Zheng