BrdU Antibody 6 Publications

Mouse Monoclonal| Catalog number: 66241-1-Ig |CloneNo.: 1B10E12

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Con: 233 μg/150 μl

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Species specificity:
human, mouse, rat

Positive IHC detected in:
mouse spleen tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
IHC : 1:200-1:1000
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2a

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Compound

Full name:
BrdU

Calculated molecular weight:

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR
Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.


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