C12orf51 Polyclonal antibody

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  WB analysis using 20609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20609-1-AP (C12orf51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

C12orf51 Polyclonal Antibody for WB, ELISA

Cat No. 20609-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HECTD4, Probable E3 ubiquitin-protein ligase HECTD4, KIAA0614, HECT-type E3 ubiquitin transferase HECTD4, HECT domain-containing protein 4

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20609-1-AP targets C12orf51 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C12orf51 fusion protein Ag14614
• Full Name: chromosome 12 open reading frame 51
• Calculated Molecular Weight: 3996 aa, 439 kDa
• Observed Molecular Weight: 500 kDa
• GenBank Accession Number: BC006270
• Gene Symbol: C12orf51
• Gene ID (NCBI): 283450
• RRID: AB_3669337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).