|Positive WB detected in||human blood tissue, human plasma|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14554-1-AP targets C1S in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C1S fusion protein Ag5977|
|Full Name||complement component 1, s subcomponent|
|Calculated molecular weight||77 kDa|
|Observed molecular weight||88 kDa|
|GenBank accession number||BC056903|
|Gene ID (NCBI)||716|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.
Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.
Int J Mol Sci
Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina
Natural antibodies and CRP drive anaphylatoxin production by urate crystals.
C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.