C1S Polyclonal antibody

C1S Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14554-1-AP


C1 esterase, C1S, Complement C1s subcomponent

Tested Applications

Positive WB detected inrat plasma, human plasma
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14554-1-AP targets C1S in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1S fusion protein Ag5977
Full Name complement component 1, s subcomponent
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC056903
Gene Symbol C1S
Gene ID (NCBI) 716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.


Product Specific Protocols
WB protocol for C1S antibody 14554-1-APDownload protocol
IHC protocol for C1S antibody 14554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Mol Sci

Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina

Authors - Anne Enzbrenner

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts

Sci Rep

Natural antibodies and CRP drive anaphylatoxin production by urate crystals.

Authors - Anne Kathrin Wessig

Cell Rep

Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.

Authors - Diana Pauly