C1S Polyclonal antibody

C1S Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14554-1-AP

Synonyms

C1 esterase, C1S, Complement C1s subcomponent



Tested Applications

Positive WB detected inhuman blood tissue, human plasma
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14554-1-AP targets C1S in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1S fusion protein Ag5977
Full Name complement component 1, s subcomponent
Calculated molecular weight 77 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC056903
Gene symbol C1S
Gene ID (NCBI) 716
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.

Protocols

Product Specific Protocols
WB protocol for C1S antibody 14554-1-APDownload protocol
IHC protocol for C1S antibody 14554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Rep

Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.

Authors - Diana Pauly
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts