Complement C6 Polyclonal antibody

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  WB analysis of human blood using 17239-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17239-1-AP (C6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 17239-1-AP

  IP result of anti-C6 (IP:17239-1-AP, 4ug; Detection:17239-1-AP 1:800) with human plasma lysate 4000ug.

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  IHC staining of mouse liver using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 40x lens).

Complement C6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17239-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

C6, Complement component C6, complement component 6

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Tested Applications

• Positive WB detected in: human blood
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: mouse liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

17239-1-AP targets Complement C6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Complement C6 fusion protein Ag11083
• Full Name: complement component 6
• Calculated Molecular Weight: 934 aa, 105 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC035723
• Gene Symbol: C6
• Gene ID (NCBI): 729
• RRID: AB_2878366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13671
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.

Publications

Species	Application	Title
human	IF	PLoS One; Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury.; Authors - Shani Zilberman-Itskovich
human	WB	Oral Dis; Analysis of Salivary Exosomal Proteins in Young Adults with Severe Periodontitis.; Authors - Xiaoyi Huang
human	IHC	Ann Diagn Pathol; The histologic and molecular correlates of liver disease in fatal COVID-19 including with alcohol use disorder; Authors - Gerard J. Nuovo
rat	IF	Ren Fail; Effect of anti-C5 antibody on recuperation from ischemia/reperfusion-induced acute kidney injury.; Authors - Shani Zilberman-Itskovich
human	IHC	Ann Diagn Pathol; The amplification of CNS damage in Alzheimer's disease due to SARS-CoV2 infection; Authors - Gerard J. Nuovo
human	IF	Nat Commun; A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells; Authors - Xue Li