C6 Polyclonal antibody

C6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17239-1-AP

Synonyms

C6, complement component 6, Complement component C6



Tested Applications

Positive WB detected inhuman blood
Positive IP detected inhuman plasma tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17239-1-AP targets C6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C6 fusion protein Ag11083
Full Name complement component 6
Calculated Molecular Weight 934 aa, 105 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC035723
Gene Symbol C6
Gene ID (NCBI) 729
RRIDAB_2878366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.

Protocols

Product Specific Protocols
WB protocol for C6 antibody 17239-1-APDownload protocol
IHC protocol for C6 antibody 17239-1-APDownload protocol
IP protocol for C6 antibody 17239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

PLoS One

Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury.

Authors - Shani Zilberman-Itskovich
humanWB

Oral Dis

Analysis of Salivary Exosomal Proteins in Young Adults with Severe Periodontitis.

Authors - Xiaoyi Huang
humanIHC

Ann Diagn Pathol

The histologic and molecular correlates of liver disease in fatal COVID-19 including with alcohol use disorder

Authors - Gerard J. Nuovo
ratIF

Ren Fail

Effect of anti-C5 antibody on recuperation from ischemia/reperfusion-induced acute kidney injury.

Authors - Shani Zilberman-Itskovich
humanIHC

Ann Diagn Pathol

The amplification of CNS damage in Alzheimer's disease due to SARS-CoV2 infection

Authors - Gerard J. Nuovo
humanIF

Nat Commun

A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells

Authors - Xue Li