• Featured Product
  • KD/KO Validated

cGAS Polyclonal antibody

cGAS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26416-1-AP

Synonyms

C6orf150, cGAS, Cyclic GMP-AMP synthase, h-cGAS, MB21D1



Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26416-1-AP targets cGAS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen cGAS fusion protein Ag23455
Full Name chromosome 6 open reading frame 150
Calculated molecular weight 496 aa, 54 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC113608
Gene symbol C6orf150
Gene ID (NCBI) 115004
RRIDAB_2880507
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Protocols

Product Specific Protocols
WB protocol for cGAS antibody 26416-1-APDownload protocol
IHC protocol for cGAS antibody 26416-1-APDownload protocol
IF protocol for cGAS antibody 26416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
humanWB,IF

Cell

Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.

Authors - Nathan Meade
WB,IF

Adv Sci (Weinh)

PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS-STING-Mediated Interferon Response.

Authors - Xinwei Geng
humanWB

Nat Commun

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Authors - Maximilian Hirschenberger
WB

Mol Cell

CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity

Authors - Guiheng Zhang
  • KO Validated
humanWB

J Clin Invest

Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.

Authors - Abhishek Jauhari

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sonia (Verified Customer) (09-12-2023)

Very clear staining of ruptured micronuclei in U2OS cells, fixed in 4% FA and permeabilised with PBS-Triton 0.5%. Primary antibody incubation was performed o/n at 4ºC in blocking buffer at 1:250.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: U2OS cells
FH

Shunbin (Verified Customer) (06-15-2023)

Produces a specific band and two other non-specific bands under the indicated conditions. The other cGAS Ab from Cell Signaling Technology produces one single band. (compare images attached

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
cGAS Antibody Western Blot validation (1:1000 dilution) in (Cat no:26416-1-AP)
FH

Zhiping (Verified Customer) (03-18-2022)

Good Ab for WB

  • Applications: Western Blot
FH

Coralie (Verified Customer) (10-20-2021)

Cytosolic staining of cGAS (forms dots, after PFA fixation).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: HFF
cGAS Antibody Immunofluorescence validation (1/100 dilution) in HFF (Cat no:26416-1-AP)