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cGAS Polyclonal antibody
cGAS Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 26416-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, THP-1 cells |
| Positive IHC detected in | human colon cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 16 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
The immunogen of 26416-1-AP is cGAS Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | cGAS fusion protein Ag23455 |
| Full Name | chromosome 6 open reading frame 150 |
| Calculated molecular weight | 496 aa, 54 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC113608 |
| Gene symbol | C6orf150 |
| Gene ID (NCBI) | 115004 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for cGAS antibody 26416-1-AP | Download protocol |
| IHC protocol for cGAS antibody 26416-1-AP | Download protocol |
| IF protocol for cGAS antibody 26416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immun Inflamm Dis Activation of the cGAS-STING signaling pathway in adenomyosis patients. | ||
Exp Eye Res The cGAS-STING signaling pathway contributes to the inflammatory response and autophagy in Aspergillus fumigatus keratitis.
| ||
FEBS Lett IFI16 regulates HTLV-1 replication through promoting HTLV-1 RTI-induced innate immune responses.
| ||
Life Sci Small molecule inhibition of cyclic GMP-AMP synthase ameliorates sepsis-induced cardiac dysfunction in mice. | ||
J Neurol Circulating cell-free mtDNA release is associated with the activation of cGAS-STING pathway and inflammation in mitochondrial diseases. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Zhiping (Verified Customer) (03-18-2022) | Good Ab for WB
|
FH Coralie (Verified Customer) (10-20-2021) | Cytosolic staining of cGAS (forms dots, after PFA fixation).
 |
