|Positive WB detected in
|HL-60 cells, THP-1 cells
|Positive IHC detected in
|human colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IHC : 1:400-1:1600
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
26416-1-AP targets cGAS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|cGAS fusion protein Ag23455
|chromosome 6 open reading frame 150
|Calculated molecular weight
|496 aa, 54 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.
Signal Transduct Target Ther
TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.
Adv Sci (Weinh)
PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS-STING-Mediated Interferon Response.
ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling
CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity
J Clin Invest
Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sonia (Verified Customer) (09-12-2023)
Very clear staining of ruptured micronuclei in U2OS cells, fixed in 4% FA and permeabilised with PBS-Triton 0.5%. Primary antibody incubation was performed o/n at 4ºC in blocking buffer at 1:250.
Shunbin (Verified Customer) (06-15-2023)
Produces a specific band and two other non-specific bands under the indicated conditions. The other cGAS Ab from Cell Signaling Technology produces one single band. (compare images attached
Zhiping (Verified Customer) (03-18-2022)
Good Ab for WB
Coralie (Verified Customer) (10-20-2021)
Cytosolic staining of cGAS (forms dots, after PFA fixation).