cGAS Polyclonal antibody

• Featured Product
• KD/KO Validated

cGAS Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 26416-1-AP

Synonyms

C6orf150, cGAS, Cyclic GMP-AMP synthase, h-cGAS, MB21D1

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Validation Data Gallery

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  WB analysis using 26416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26416-1-AP (C6orf150 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 26416-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26416-1-AP (C6orf150 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26416-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26416-1-AP (C6orf150 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells
• Positive IHC detected in: human colon cancer tissue, mouse ovary tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

The immunogen of 26416-1-AP is cGAS Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: cGAS fusion protein Ag23455
• Full Name: chromosome 6 open reading frame 150
• Calculated molecular weight: 496 aa, 54 kDa
• Observed molecular weight: 58 kDa
• GenBank accession number: BC113608
• Gene symbol: C6orf150
• Gene ID (NCBI): 115004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Publications

Species	Application	Title
human	IHC	Immun Inflamm Dis; Activation of the cGAS-STING signaling pathway in adenomyosis patients.; Authors - Yun Lin
human	WB	Exp Eye Res; The cGAS-STING signaling pathway contributes to the inflammatory response and autophagy in Aspergillus fumigatus keratitis.; Authors - Fang Han ; KD Validated
human	WB	FEBS Lett; IFI16 regulates HTLV-1 replication through promoting HTLV-1 RTI-induced innate immune responses.; Authors - Bo Yang ; KD Validated
mouse	WB	Life Sci; Small molecule inhibition of cyclic GMP-AMP synthase ameliorates sepsis-induced cardiac dysfunction in mice.; Authors - Qingqin Xu
human	WB	J Virol; mTOR Dysregulation by Vaccinia Virus F17 Controls Multiple Processes with Varying Roles in Infection.; Authors - Nathan Meade
human	WB	J Virol; Restriction of HCMV replication by ISG15, a host effector regulated by cGAS-STING dsDNA sensing.; Authors - Christopher Bianco ; KD Validated