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  • KD/KO Validated

cGAS Polyclonal antibody

cGAS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26416-1-AP

Synonyms

C6orf150, cGAS, Cyclic GMP-AMP synthase, h-cGAS, MB21D1



Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26416-1-AP is cGAS Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen cGAS fusion protein Ag23455
Full Name chromosome 6 open reading frame 150
Calculated molecular weight 496 aa, 54 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC113608
Gene symbol C6orf150
Gene ID (NCBI) 115004
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Protocols

Product Specific Protocols
WB protocol for cGAS antibody 26416-1-APDownload protocol
IHC protocol for cGAS antibody 26416-1-APDownload protocol
IF protocol for cGAS antibody 26416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Rep

Recruitment of DNA to tumor-derived microvesicles.

Authors - James W Clancy
humanIHC

Immun Inflamm Dis

Activation of the cGAS-STING signaling pathway in adenomyosis patients.

Authors - Yun Lin
humanWB

Exp Eye Res

The cGAS-STING signaling pathway contributes to the inflammatory response and autophagy in Aspergillus fumigatus keratitis.

Authors - Fang Han
  • KD Validated
humanWB

FEBS Lett

IFI16 regulates HTLV-1 replication through promoting HTLV-1 RTI-induced innate immune responses.

Authors - Bo Yang
  • KD Validated
mouseWB

Life Sci

Small molecule inhibition of cyclic GMP-AMP synthase ameliorates sepsis-induced cardiac dysfunction in mice.

Authors - Qingqin Xu
humanWB,IF

J Neurol

Circulating cell-free mtDNA release is associated with the activation of cGAS-STING pathway and inflammation in mitochondrial diseases.

Authors - Xutong Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zhiping (Verified Customer) (03-18-2022)

Good Ab for WB

  • Applications: Western Blot
FH

Coralie (Verified Customer) (10-20-2021)

Cytosolic staining of cGAS (forms dots, after PFA fixation).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: HFF
cGAS Antibody Immunofluorescence validation (1/100 dilution) in HFF (Cat no:26416-1-AP)