C7orf63 Polyclonal antibody

• 

  WB analysis of NIH/3T3 using 24001-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24001-1-AP (C7orf63 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 24001-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24001-1-AP (C7orf63 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24001-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24001-1-AP (C7orf63 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 24001-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using C7orf63 antibody (24001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 24001-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using C7orf63 antibody (24001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat testis using 24001-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using C7orf63 antibody (24001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C7orf63 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 24001-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CFAP69, Cilia- and flagella-associated protein 69

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24001-1-AP targets C7orf63 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C7orf63 fusion protein Ag21205
• Full Name: chromosome 7 open reading frame 63
• Calculated Molecular Weight: 941 aa, 106 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC132771
• Gene Symbol: C7orf63
• Gene ID (NCBI): 79846
• RRID: AB_3085719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: A5D8W1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.